Hypoxia-induced carbonic anhydrase mediated dorsal horn neuron activation and induction of neuropathic pain

1. Introduction

Chronic pain is a significant burden faced by patients, with pain not restricted to a single condition but developing because of an array of differing health and disease-related afflictions. Forty-three percent of the United Kingdom17 and 25% of the global population20 suffer from chronic pain, which can arise either idiopathically, as a consequence of another disease (eg, diabetes68), or as a consequence of therapyo (eg, chemotherapy46). Neuropathic pain arises due to damage to the somatosensory sensory system with the modulation of intrinsic sensory neural circuits fundamental to the maintenance of nociception4 and presented as a hypersensitivity to evoked sensory stimuli (eg, allodynia or hyperalgesia) as well as ongoing pain18,30. There is a severe lack of effective analgesic management in the clinic.6

Dorsal horn neurons possess the ability to adapt to neuronal stress through key mechanisms that underlie maladaptive plasticity32 A balance between excitatory and inhibitory processing is maintained, but during chronic pain, excitatory pathways predominate40 with GABAergic excitation of a contributing factor.33,51 How these sensory neuronal networks alter in times of stress is unknown. Neural tissues are susceptible to depleted oxygen availability, although they can reprogram metabolic pathways to support continued neuronal function.29 Perturbed vascular function accompanies the development of neurodegenerative conditions such as Alzheimer disease,45 and a causal relationship between peripheral limb ischaemia and onset of pain has been shown.22,39 Previous work has explored systems' level modulation of blood flow and the resulting impact on nociceptive processing and modulation of pain behaviours.7,70 Reduced dorsal horn spinal cord blood flow in a rodent model of diabetes, potentially due to vascular rarefaction, is associated with the onset of pain hypersensitivity.70 Vascular integrity is mediated by the vascular endothelial growth factor (VEGF) family of proteins, signalling through VEGF receptor 2 (VEGFR2). Vascular endothelial growth factor is a master regulator of angiogenic function, with fluctuations in VEGF expression associated with distinct endothelial cell activity relating to physiological function and pathogenesis. High VEGF expression and VEGFR2-mediated signalling drives aberrant vascular expansion,24,65 whereas diminished VEGFR2 signalling leads to vascular pruning.31 Interestingly, a systemic endothelial specific knockout of VEGFR2, predicted to reduce vascular density in the spinal cord, reduced nociceptive behavioural hypersensitivity in an animal model of arthritis, by reducing spinal cord microglial activation.7 We, therefore, set out to determine whether vascular rarefaction in the spinal cord per se initiates neuropathic pain phenotypes.

Here we show that the spinal cord microvasculature is critical in maintaining sensory neuronal modulation and demonstrate that alterations in vascular support to the dorsal horn contribute to diabetic neuropathic pain development. Type I diabetic and transgenic rodent models (endothelial-specific VEGFR2 knockout) of reduced spinal cord vascularity demonstrate that localised vascular degeneration induces a hypoxic microenvironment in the dorsal horn, leading to neuron activation and initiation of neuropathic pain. The initiation of neuropathic pain is dependent on the hypoxia response, in particular the upregulation of hypoxia inducible genes and the resultant change in bicarbonate metabolism, which can be prevented by carbonic anhydrase inhibition. These results show a potential new therapeutic strategy for analgesia development.

2. Methods 2.1. Ethical approval and animals used

All experiments using animals were performed in accordance with the UK Home Office Animals (Scientific Procedures) Act 1986 and EU Directive 2010/63/EU, in line with the ARRIVE guidelines, with experimental design and procedures reviewed by the local Animal Welfare and Ethics Review Boards (University of Nottingham and Nottingham Trent University). Animals had ad libitum access to standard chow and were housed under 12:12 hours light:dark conditions.

2.2. Rodent models of diabetes

Sprague–Dawley female rats (∼250 g) and Db/db mice were purchased from Charles River. Db/db mice are rodent models of type II diabetes, which have impaired leptin receptor signalling to induce obesity and hyperglycaemia (Charles River; vs lean controls). Streptozotocin-induced rodent model of type 1 diabetes; Sprague–Dawley female rats were used and treated with either vehicle (saline; naive Group, n = 15) or streptozotocin (50 mg/kg; STZ in saline; n = 15) administered by intraperitoneal injection. Hyperglycaemia was determined as >15 mmol in samples of blood extracted by tail prick (end of study week 8). Under isoflurane anaesthesia (∼2% oxygen), one third of an insulin pellet (LinShin, Canada) was implanted in the scruff of the neck using a trocar, in STZ-treated animals. Experimental group animal body weights and blood glucose measurements were recorded during the study. Measures (mean ± SEM) recorded at the end of the study (week 8) are presented below: blood glucose (mmol/dL): naive = 7.44 ± 0.29, diabetic =28.5 ± 1.82, animal body weight (grams): naive = 330.6 ± 4.49, and diabetic = 284.2 ± 4.86.

2.3. Transgenic mice

Tie2CreERT2 mice (European Mutant Mouse Archive Tg [Tek-cre/ERT2] 1Arnd) were interbred with homozygous vegfr2fl/fl mice (as previously described).2,7,63 This generates tamoxifen inducible endothelial-specific vegfr2 knockout adult mice. Mice (both genders) used were vegfr2fl/fl and were either Tie2CreERT2 negative (n = 83, termed WT) or positive (n = 83, termed VEGFR2CreERT2). Mice were treated with either intraperitoneal injection of 1 mg tamoxifen per mouse (10% ethanol in sunflower oil) once daily for 5 days consecutively (n = 116)7,70 referred to as VEGFR2ECKO or a single intrathecal injection of 1 μM 4-hydroxytamoxifen (OHT; 10% ethanol in sunflower oil) (n = 34) referred to as VEGFR2scECKO. The remaining 16 mice were used for endothelial cell culture. All transgenic animals were taken 8 days after first tamoxifen or OHT injection. All intrathecal injections were performed under brief isoflurane anaesthesia (∼2% oxygen).

2.4. Chemically induced hypoxia

For the hypoxia mimetic study, 1 mM dimethyloxalylglycine (DMOG, in PBS, Tocris, 22 mice) or vehicle (16 mice) was intrathecally injected into C57Bl6 adult male mice (total 38) under recovery anaesthesia (isoflurane ∼2% oxygen). Acetazolamide (ACZ) was delivered through intraperitoneal injection (40 mg/kg, in 1% DMSO in PBS, Tocris).

2.5. Intravital imaging

C57Bl6 male mice (∼30 g; n = 12) were deeply terminally anaesthetised and anaesthesia maintained (ip ketamine and medetomidine). Body temperature was maintained (∼ 37°C) using a feedback-controlled heating blanket with a rectal probe. Midline incision was made along the thoracic–lumbar region of the mouse, and muscle was dissected from the vertebrae. A laminectomy was performed between thoracic (T10) and lumbar vertebra (L5) to expose the lumbar spinal cord. In house stainless steel spinal vertebrae clamps were used to stabilise the vertebrae while an in house window chamber was attached to the spinal cord vertebra.16 The spinal cord hydration was maintained with physiological saline before the application of Kwik-Sil silicone elastomer (World Precision Instruments) and a 5 mm coverslip number 1 (Harvard Apparatus). Following this, the mouse was placed on to the confocal microscope (Leica SPE) and images were captured by 10x objective. Mice were administered Alexa Fluor 555 Conjugated Wheat Germ Agglutinin (1 mg/mL, Thermo Scientific) by tail vein injection. On identification of the endothelium, sodium fluorescein (i.p. 100 mg/mL) was administered intraperitoneally.3 Image acquisition began immediately after sodium fluorescein injection. Spinal cords were treated with either vehicle (PBS) or 2.5 nM VEGF-A165a with or without VEGFR2 inhibitor ZM323881 (100 nM) for 5 minutes before fluorescein injection (as described by Wardlaw et al.72). Sodium fluorescein permeability was measured across multiple microvessels, as previously described in the mesentery,9 which involved determining a region of interest extending across the vessel width and encompassing the region outside the vessel wall with solute permeability determined. Animals were killed by anaesthetic overdose on completion of the study.

2.6. Nociceptive behaviour

Animals were habituated to the testing environment before nociceptive behavioural experimentation, which were performed as previously described.70 Mechanical withdrawal thresholds were determined by application of von Frey (vF) monofilaments to the hind paw plantar surface. von Frey filaments of increasing diameter were applied (a maximum of 5 seconds or until paw withdrawal), with each vF filament applied a total of 5 times to generate force response curves. Withdrawal frequencies of 50% at each weight were used to determine the mechanical withdrawal threshold (grams).8 The Hargreaves test was performed to measure thermal nociceptive behaviour.21 A radiant heat source was focused on the plantar surface of the hind paw. The time taken for the mouse to withdraw their paws was recorded. The latency to hind paw response was measured 3 times. For motor behavioural assessment, mice were placed in Perspex enclosures and were left undisturbed for 5 minutes while being video recorded to explore the environment.48 After experimentation, animals were returned to housing cages and data were analysed offline. All assays were undertaken by observers blinded to treatment or genotype. Animals were killed by perfusion fixation.

Animals that had been terminally anaesthetised with sodium pentobarbital (60 mg/kg, Sigma-Aldrich) and transcardially perfused with PBS followed by 4% PFA (pH7.4) had tissue dissected, cryoprotected (30% sucrose), and stored at −80°C until spinal cords were cryosectioned (40 μm thickness).70 Slides were incubated in primary antibodies (Table 1) in blocking solution (5% bovine serum albumin, 10% fetal calf serum, and 0.2% Triton-X) at 4°C for 72 hours and then washed in PBS. Slides were subsequently incubated in secondary antibodies in PBS + 0.2% Triton X-100 at room temperature for 2 hours. Confocal microscopy of the dorsal horn of the lumbar region of the spinal cord was performed using a Leica SPE confocal microscope.

Table 1 - List of primary and secondary antibodies for immunofluorescence. Biotin-conjugated isolectin B4 (IB4; 1:100, Sigma Aldrich) PECAM (1:100, Santacruz) Rabbit anti-CD31 (1:00, Abcam) Chicken anti-NeuN (1:500, Synaptic systems) Chicken anti Map2 (1:500, Millipore) Rabbit FI10:13C-conjugated antihypoxyprobe (1:200, Hypoxyprobe, Omni kit) Mouse anti-CD31 (1:100, Abcam) Rabbit anti-fos (1:100, Santa Cruz) Mouse anti-NeuN (1:200, Millipore) Guinea pig anti-NeuN (1:100, Synaptic Systems) Mouse anti-Hif1a (1:100, BD Bioscience) Rabbit anti-collagen IV (1:200; Abcam) Glut3 (1 in 200: Alomone) Biotinylated anti-rabbit IgG (1:500, Jackson Laboratories) (for-cfos) Secondary antibodies (1:500, all Invitrogen, United Kingdom) used were Alexa Fluor 488-conjugated chicken anti-mouse, Alexa Fluor 555-conjugated donkey anti-rabbit, and streptavidin-conjugated Alexa Fluor-555

GLUT3, glucose transporter 3; HIF1α, hypoxia-inducible factor 1α.


2.7. Hypoxyprobe assay

Hypoxyprobe staining was performed as outlined in the manufacturer's instructions (Hypoxyprobe Omni Kit).58 Fiveik Tie2CreERT2 vegfr2fl/fl (VEGFR2scECKO) mice and 5 vegfr2fl/fl (WT) were used 8 days after intrathecal injection of hydroxytamoxifen was used, as well as 5 STZ-induced diabetic and 5 vehicle-treated naive rats were used. Experimental animals were administered 60 mg/kg hypoxyprobe by intraperitoneal injection. Animals were terminally anaesthetised with sodium pentobarbital (60 mg/kg, Sigma-Aldrich) 30 minutes later and transcardially perfused with PBS followed by 4% paraformaldehyde (PFA; pH7.4). Lumbar spinal cords were extracted and cryosectioned as outlined above. Anti-pimonidazole rabbit antisera (PAb2627AP) was added to slides at 1 in 200 dilution overnight at 4°C and subsequently incubated in Alexafluor-555-conjugated donkey anti-rabbit in PBS + 0.2% Triton X-100 at room temperature for 2 hours. Confocal imaging of the dorsal horn of the lumbar spinal cord was performed on a Leica TCS SPE confocal microscope.

2.8. Tetramethylrhodamine isothiocyanate dextran perfusion assay

Five Tie2CreERT2 vegfr2fl/fl (VEGFR2scECKO) mice and 5 vegfr2fl/fl (WT) mice were terminally anaesthetised 8 days after intrathecal administration of 1 μM hydroxytamoxifen; and anaesthesia was maintained with sodium pentobarbital (60 mg/kg, Sigma-Aldrich). The external jugular vein was cannulated, and 1 mg per 20 g tetramethylrhodamine isothiocyanate (TRITC) dextran (76 Mw; Sigma-Aldrich) was injected.47,69 Mice were monitored for 30 minutes after TRITC dextran infusion. Subsequently animals were transcardially perfused with 4% PFA (pH 7.4), and lumbar spinal cords were extracted, and samples were prepared for immunofluorescent confocal imaging as outlined previously.

2.9. Immunocytochemistry

Spinal cord neurons were isolated as outlined above and plated on glass coverslips coated in poly-l-lysine (24 hours, Sigma-Aldrich) followed by laminin (5 mg/mL 2 hours, Sigma-Aldrich). They were cultured in media for 24 hours and subsequently washed in PBS, before incubation in 4% PFA for 15 minutes at room temperature. Neurons were permeabilised with 0.4% Triton X-100 in PBS for 15 minutes at room temperature. Cells were incubated in blocking solution (5% bovine serum albumin, 10% fetal calf serum, and 0.2% Triton-X), mixed gently, and incubated for 30 minutes at room temperature. Cells were incubated in primary antibodies for identification of neurons (chicken anti-MAP2, SySy) overnight at 4OC. Cells were subsequently washed in PBS and secondary antibodies (Alexa Fluor 555-conjugated donkey anti-mouse and Alexa Fluor 488-conjugated donkey anti-chicken) added for 2 hours at room temperature. Glass coverslips were mounted on slides using Vector Shield (H-1000, Vector Labs).

2.10. qPCR method

Whole lumbar spinal cord lysates (5 Tie2CreERT2 vegfr2fl/fl [VEGFR2scECKO] mice and 5 vegfr2fl/fl [WT] mice) and isolated lumbar spinal cord neurons (5 C57.bl6 mice) were used (killed via cervical dislocation) to extract RNA in experimental groups using TRIzol reagent (Invitrogen) from 10 C57.bl6 mice. One microgram RNA was reverse transcribed to cDNA using PrimeScriptRT reagent kit (TaKaRa, RR037A). Quantitative PCR was performed using a LightCycler 480 SYBR Green I Mastermix (Roche 04707516001) following the manufacturer's instructions. All primers were obtained from Eurofins and outlined in Table 2. All other primers were obtained from Sigma-Aldrich. The reference gene used was β-actin. The primer sequences were as follows (5′-3′).

Table 2 - List of primers used. B-actin F – 5′ ATTGCCAATGAGCGGTTC-3′ B-actin R–5′ GGATGCCACAGGACTCCA-3′ CD31 F–5′ GAAATGCTCTCGAAGCCCAG-3′ CD31 R–5′ ACCTCGAGAGTCTGGAAGTC-3′ VE-cadherin F–5′ TCCCTGGACTATGAAGTCAT-3′ VE-cadherin R–5′ GAAGACAGGGGGCTCATCCA-3′ VEGFR2 F–5′ GGATCTGAAAAGACGCTTGG-3′ VEGFR2 R–5′ TGCTCCAAGGTCAGGAAGTC-3′ Carbonic anhydrase II-F–5′ CAAGCACAACGGACCAGA-3′ Carbonic anhydrase II-R–5′ ATGAGCAGAGGCTGTAGG-3′ Carbonic anhydrase VII F–5′ CAATGACAGTGATGACAGAA-3′ Carbonic anhydrase VII R–5′ TCCAGTGAACCAGATGTAG-3′ Carbonic anhydrase F–5′ CTGAAGACAGGATGGAGAAG-3′ Carbonic anhydrase R–5′ GCAGAGTGCGGCAGAATG-3′ GLUT1 F–5′ CATCCTTATTGCCCAGGTGTTT-3′ GLUT1 R–5′ GAAGACGACACTGAGCAGCAGA-3′ GLUT3 F: 5′ GGAGGAAGACCAAGCTACAGAG-3′ GLUT3 R–5′ GAGCTCCAGCACAGTCACCT-3′ HIF1-alpha F–5′ TGCTCATCAGTTGCCACTTC-3′ HIF1-alpha R–5′ TCCTCACACGCAAATAGCTG-3′

GLUT3, glucose transporter 3; HIF1α, hypoxia-inducible factor 1α; VEGF, vascular endothelial growth factor; VEGFR2, VEGF receptor 2.


2.11. Multielectrode arrays

The mouse lumbar spinal cord was extracted from 5 C57.Bl6 male mice (Charles River; killed by cervical dislocation) and maintained in neurobasal media (supplemented with B27 and L-Glutamine, Invitrogen). Lumbar spinal cord tissue was cut to 400 μm thickness and incubated on high-density microelectrode arrays (3Brain, 4096 electrodes). The neuronal electrical activity was recorded digitally by the Biocam X after 24 hours treatment with either vehicle or 1 mM DMOG application. Data were recorded offline using BrainWave analysis software.

2.12. Western blotting

Protein was extracted from lumbar spinal cord tissue collected as above and L3-L5 dorsal root ganglion as previously described8,70 from experimental animals (killed by cervical dislocation)–experimental groups (VEGFR2KO; 5 Tie2CreERT2 vegfr2fl/fl [VEGFR2scECKO] mice and 5 vegfr2fl/fl [WT] mice as well as intrathecal DMOG; 5 saline-treated mice and 5 DMOG-treated mice) studies compared with vehicle experimental controls as above. In additional experiments, protein lysate samples were also extracted from in vitro lumbar spinal cord slices prepared from 6 C57bl6 male adult mice and slices maintained in Neurobasal media (supplemented with L-Glutamine, Invitrogen) and exposed to vehicle or 1 mM DMOG for 24 hours. Tissue was lysed using RIPA buffer (ThermoFisher) containing protease inhibitor cocktail (20 μL/mL buffer; 1 mM phenylmethylsulfonyl fluoride, 10 mM sodium orthovanadate, and 50 mM sodium fluoride, Sigma Aldrich). Equal protein lysate concentrations were loaded on a 4% to 20% precast Mini-Protean gradient TGX gel (Biorad), separated by SDS-PAGE electrophoresis, and transferred using Trans-blot turbo transfer system (Bio-Rad) to a PVDF membrane. The membrane was incubated in 5% BSA in tris-buffered saline-Tween 0.1% (TBST) for 1 hour at room temperature, followed by incubation with primary antibodies (Table 3) overnight at 4°C. The membrane was then incubated in secondary antibodies in TBST-0.1% to 1% BSA. Membranes were washed 3 times with TBST and visualised on the Licor Odyssey Fc.

Table 3 - List of primary and secondary antibodies for Western blotting. HIF1-alpha anti-mouse (1 in 100, BD Bioscience) Actin–Rabbit polyclonal anti-Beta-Actin (1 in 100 Santa Cruz) Actin–Goat polyclonal anti-Beta-Actin (1 in 200 Santa Cruz) Tubulin–anti-rabbit anti-α tubulin (1 in 1000, Cell Signalling, #2144) ZO-1–rabbit anti-ZO1 (1 in 500, Invitrogen, Cat #61-7300) Occludin–rabbit anti‐occludin (5 μg/mL, Invitrogen) CD-31 mouse monoclonal anti-CD31 (2 μg/mL, Abcam; AB24590), CA-7 rabbit (1 in 500, cell signalling, #PA5-103703) VEGFR2–rabbit anti VEGFR2 (1 in 500, Cell Signalling, #2479) Licor donkey anti-goat, anti-rabbit, and anti-mouse antibodies 1:10,000

HIF1α, hypoxia-inducible factor 1α; VEGF, vascular endothelial growth factor; VEGFR2, VEGF receptor 2.


2.13. Spinal cord endothelial cell culture

Whole lumbar spinal cords were dissected from 16 Tie2CreERT2 vegfr2fl/fl mice (culled by cervical dislocation), and spinal cord endothelial cells were isolated as previously described.70 Spinal cords were incubated in 1.25% collagenase in endothelial cell media (M199 media, 60 mg/mL endothelial cell growth supplement and 50 μg/mL Heparin; Sigma-Aldrich) for 30 minutes at 37°C, 5% oxygen. The cell suspension was centrifuged at 240 g for 5 minutes. The supernatant was discarded, and the pellet was resuspended in media with an equal volume of 1% BSA and centrifuged at 1200 g for 20 minutes. The BSA or myelin fraction was aspirated and discarded. The remaining cell pellet resuspended in endothelial cell media and plated onto a 96-well sterile plates coated with 1% gelatin. On 80% cell confluency, spinal cord endothelial cells were treated for 24 hours with either media supplemented with either vehicle (10% ethanol in sunflower oil) or OHT. Cell viability was determined with Neutral Red (Sigma-Aldrich).

2.14. Spinal-isolated neuronal cell culture

Spinal cord neurons were dissected as described by Brewer and Torricelli.10 Whole spinal cords were isolated from 10 C57Bl6 mice (Charles River; killed by cervical dislocation) and digested in papain (2 mg/mL, Worthington, cat. no. 3119) for 30 minutes at 30°C. The cell suspension was triturated using Pasteur pipette. To purify neurons, a density gradient separation preparation was performed using OptiPrep (Sigma-Aldrich) density gradient. The neuronal containing fraction was collected and centrifuged (1100 rpm 2 minutes) to obtain a pellet. The pellet was resuspended in Neurobasal media (supplemented with B27 and L-Glutamine, Invitrogen) and plated on sterile 6-well or 24-well plates coated with poly-D-Lysine (sterile lyophilized 135 kD; Sigma) and 1 mg/mL laminin. Cells were exposed to hypoxia (1% O2) or normoxia for 24 hours.

2.15. Statistical analysis

All data are represented as mean ± SEM stated, and the experimenter was blinded to group treatment during experimental procedures. Data were acquired or quantified using Microsoft Excel and GraphPad Prism 8. Quantification of immunofluorescence was performed by obtaining 5 random images from 5 random nonsequential sections (Z stacks) per animal and a mean value calculated per animal. Images were analysed using ImageJ (https://imagej.nih.gov/ij/) or Imaris 8.11 (Bitplane) to determine vessel diameter and volume. The dorsal horn neuronal number was determined according to lamina I-V as previously characterised.23 Densitometry analysis of Western blot was performed using gel quantification plugin in ImageJ. The mean fluorescence intensity was measured over time enabling visualisation of vessel perfusion and permeability. Nociceptive behaviour and the neuron number in the dorsal horn of the spinal cord were analysed using 2-way ANOVA with post hoc analysis. Western blot densitometry, QPCR, MEA recordings, and TRITC dextran perfusion were analysed using the Mann–Whitney U test. Video analysis of rodent motor behaviour was performed using MATLAB48 and analysed using the Mann–Whitney U test.

3. Results 3.1. Dorsal horn hypoxia induction

To determine whether increased diabetic neuropathic pain was associated with vascular loss and hypoxia, we used a rat model of type 1 (streptozotocin induced and insulin supplemented) that led to the development of mechanical (Fig. 1A) and heat (Fig. 1B) nociceptive behavioural hypersensitivity. This was associated with an increase in collagen sleeves (collagen positive microvessels, negative for IB4-labelled endothelium) in diabetic animals compared with age-matched vehicle control animals (Figs. 1C and D) indicative of loss of endothelium and vascular rarefaction. In addition, there was a significant increase in the number of hypoxic dorsal horn neurons (labelled with hypoxyprobe) in diabetic animals when compared with age-matched vehicle control animals (Figs. 1E and F; supplemental Figure 1A and 1B, available at https://links.lww.com/PAIN/B597) and a small decrease in the total number of identified neurons in the dorsal horn (NeuN labelled; Figs. 1G–I). These results indicate that hypoxia caused by loss of blood vessels could play a part in induction of diabetic neuropathic pain.

F1Figure 1.:

Dorsal horn hypoxia develops in a rodent model of type 1 diabetic neuropathic pain. Type 1 diabetes was induced in rats by intraperitoneal injection of streptozotocin supplemented with insulin. Diabetic rodents developed (A) decreases in mechanical withdrawal threshold and (B) reduced latencies to heat withdrawal compared with age-matched control animals (Naive). (C) Cryosections of the dorsal horn from naive and diabetic experimental groups were stained for basement membrane (Collagen IV) and endothelium (IB4). (D) In the diabetic dorsal horn, there was reduction in endothelial coverage of the basement membrane (ie, increased sleeves) compared with age-matched naive animals (n = 5 per group, ** P < 0.01 unpaired t Test). (E) Representative confocal microscopy images of hypoxyprobe (HP)-positive neurons (NeuN labelled) in the dorsal horn of the spinal cord in naive and diabetic cryosections (colabelled with NeuN, scale bar = 50 μm). (F) Mapping of the dorsal horn laminae demonstrates increased hypoxyprobe positive neurons or lamina in diabetic animals when compared with naive control animals (*P < 0.05, ***P < 0.001 2-way ANOVA with Tukey multiple comparison, n = 5 per group). (G) In the diabetic rat dorsal horn, there were reductions in neuronal number in lamina 2 and 3 of the dorsal horn when compared with naive Sprague–Dawley female rats controls (*P < 0.05 2-ay ANOVA with Tukey multiple comparison, n = 10 per group). (H) Representative image of images acquired after omission of primary antibody; negative control and mapping of (I) dorsal horn lamina.

We have previously shown that VEGFR2ECKO animals had no change in nociceptive processing compared with wild type animals and were actually resistant to hyperalgesia and allodynia induced by induction of arthritis. To determine whether these mice had a change in vessel density, hypoxia, and cell number, we stained the spinal cord of these animals after knockout induction with tamoxifen. VEGFR2ECKO animals showed an increase in the number of collagen sleeves (decrease in endothelial covered collagen positive vessels) compared with age-matched litter mate control wild type animals (Figs. 2A and B), indicating vascular rarefaction. Although VEGFR2ECKO did not affect the number of neurons (NeuN labelled) in the dorsal horn across lamina 1-5 (supplemental Figure 2A–C, available at https://links.lww.com/PAIN/B597). There was a significantly increase in the number of hypoxyprobe-labelled neurons in the dorsal horn compared with control animals (Figs. 2C and D) demonstrating hypoxia as a consequence of VEGFR2ECKO. VEGFR2ECKO mice had a lower withdrawal latency to heat when compared with WT mice (Fig. 2E).

F2Figure 2.:

Vascular endothelial growth factor modulation of the spinal cord blood vasculature. (A) Dorsal horn cryosections were imaged by confocal microscopy to identify microvessels (Collagen IV–basement membrane–and IB4–endothelium, scale bar = 50 μm) in WT and VEGFR2ECKO mice, a systemic transgenic model that was induced with intraperitoneal administration of tamoxifen. (B) In VEGFR2ECKO mice, there was a decreased percentage of microvessels positive for both endothelium (IB4) and collagen IV (ie, increased sleeves) compared with WT mice (n = 5 per group, ** P < 0.01 unpaired t test). Both WT and VEGFR2ECKO mice were treated by intraperitoneal injection of hypoxyprobe. (C) Confocal microscopy of the dorsal horn was used to identify hypoxyprobe (HP)-labelled neurons (colabelled with NeuN, scale bar = 50 μm) in WT and VEGFR2ECKO mice. (D) VEGFR2ECKO mice had increased percentage of hypoxic neurons across dorsal horn laminae 1-5 compared with WT mice (*P < 0.05, **P < 0.01, ***P < 0.001 2-way ANOVA with Tukey multiple comparison, n = 5 per group). (E) Heat hypersensitivity developed in VEGF2ECKO mice when compared with WT controls (***P < 0.001 2-way ANOVA with Tukey multiple comparison, n = 10 per group). (F) To confirm VEGFR2 signalling in the spinal cord, microvessels were identified in the dorsal horn of the lumbar region of the spinal cord in C57 Bl6 mice. 100 mg/mL sodium fluorescein was administered by intraperitoneal injection, with data acquired by time lapse confocal imaging allowing for recording of solute permeability (Ps, scale bar = 20 μm). Representative images of fluorescein-filled microvessels in the spinal cord from experimental groups—PBS control and VEGF-A165a (VEGF-A65a, 2.5 nM) with either vehicle (0.01% DMSO in saline) or VEGFR2-specific antagonist ZM323221 (100 nM). (G) VEGF-A65a treatment led to increased fluorescein leakage from the dorsal horn microvessels (**P < 0.01 1-way ANOVA with Tukey multiple comparison, PBS n = 5 and VEGF-A65a +vehicle n = 6). Treatment with ZM323881 attenuated VEGF-A165–induced vascular leakage (**P < 0.01 1-way ANOVA with Tukey multiple comparison, VEGF-A165 + ZM323881 n = 7). VEGF, vascular endothelial growth factor; VEGFR2, VEGF receptor 2.

Blood vessel survival has previously been shown to require endogenous VEGFR2 activation in tissues including the oesophagus, skin, and salivary gland as well as in other tissues.27,34 However, it is not known whether VEGFR2 signalling is required for vessels in the spinal cord, which form a part of the blood–brain barrier. We therefore sought to determine whether spinal cord VEGFR2 signalling is intact in spinal cord microvessels. VEGF-A165a was administered directly to the dorsal horn of the spinal cord and vascular permeability measured using intravital imaging. VEGF-A165 increased solute permeability of the blood–spinal cord barrier when compared with vehicle control (Figs. 2F and G) which was blocked by treatment with VEGFR2 inhibitor ZM323881 (Figs. 2F and G).

3.2. Onset of chronic pain due to the induction of spinal cord restricted dorsal horn vasculopathy

The previous explored experimental models were systemic rodent models of vasculopathy, which means that pain processing could be altered in both the brain, and peripherally which could affect sensitivity. To determine whether spinal cord vascularity per se could regulate nociceptive processing, we developed a refined model that induced vasculopathy restricted to the spinal cord. Endothelial cells (CD31 positive) were extracted from WT and VEGFR2CreERT2 mice spinal cords (Fig. 3A). Cell viability was determined after OHT treatment. In a concentration-dependent manner, OHT treatment led to a significant reduction in endothelial cell viability in VEGFR2CreERT2 mice spinal cord endothelial cells when compared with WT mice spinal cord endothelial cells (Fig. 3B). To determine whether this was also apparent in vivo, spinal cord endothelial-specific VEGFR2 knockout (VEGFR2scECKO) mice were generated by intrathecal injection of OHT into the lumbar enlargement. This was confirmed by a reduction in VEGFR2 expression (Fig. 3C QPCR quantification and Fig. 3G. immunofluorescence) in the spinal cord of VEGFR2scECKO mice when compared with WT mice. In addition, endothelial markers VE-Cadherin (Fig. 3D), CD31 (Fig. 3E), and glucose transporter 1 (GLUT1, Fig. 3F) were also reduced in the spinal cord of VEGFR2scECKO mice when compared with WT mice. Furthermore, VEGFR2scECKO mice had reduced protein expression of endothelial markers (representative Western blot, Fig. 3H, and quantification of ZO1 [Fig. 3I], CD31 [Fig. 3J], and occluding [Fig. 3K]) in the spinal cord. Notably, there was no change in protein expression of these endothelial markers (ZO-1 and CD31) in the lumbar 3- 5 dorsal root ganglia of either WT or VEGFR2scECKO mice, showing that this induction was limited to the spinal cord (supplemental Figure 3, available at https://links.lww.com/PAIN/B597).

F3Figure 3.:

VEGFR2 knockout in the spinal cord induces localized vasculopathy. (A) Spinal cord endothelial cells (NG2−ve and CD31+ve) were isolated from WT and VEGFR2CreERT2 transgenic mice (scale bar = 10 μm), and (B) cell viability was determined after treatment with hydroxytamoxifen (OHT). Endothelial cells extracted from WT mice were unaffected by OHT treatment, whereas those from VEGFR2CreERT2 mice demonstrated a concentration dependent reduction in cell viability(*P < 0.05, ***P < 0.001 2-way ANOVA with Tukey multiple comparison, n = 3 per group). (C) Intrathecal injection of 1 μM OHT into the lumbar region of the spinal cord of VEGFR2CreERT2 mice (VEGFR2scECKO) led to reduced VEGFR2 mRNA expression in the lumbar spinal cord compared with similarly treated WT mice. There were reductions in mRNA expression of endothelial-specific markers (D) VE-Cadherin, (E) CD31, and (F) GLUT1 (*P < 0.05, **P < 0.01, unpaired t test, n = 5 per group). (G) VEGFR2 immunoreactivity was reduced in CD31-labelled microvessels (scale bar = 50 μm) in the dorsal horn of VEGFR2scECKO mice. (H) Representative immunoblots show reductions in protein expression of endothelial markers in the lumbar region of the spinal cord from VEGFR2scECKO mice. Densitometric quantification demonstrates reduced protein expression of (I) ZO1, (J) CD31, and (K) occludin in VEGFR2scECKO mice (**P < 0.01, unpaired t test, n = 5 per group). VEGF, vascular endothelial growth factor; VEGFR2, VEGF receptor 2.

Intrathecal OHT treatment led to significant vascular degeneration (Fig. 4A) of the microvasculature in the dorsal horn of VEGFR2scECKO mice, shown as reduced vessel diameter (Fig. 4B) and decreased volume of total vessel coverage (Fig. 4C). In addition, there was a reduction in dorsal horn vessel perfusion in VEGFR2scECKO mice indicated by decreased TRITC-Dextran–labelled vessels (Fig. 4D). This reduction in vessel perfusion was accompanied by an increase in hypoxyprobe-labelled neurons (Fig. 4F, hypoxyprobe labelled with NeuN, supplemental Figure 4A, available at https://links.lww.com/PAIN/B597) in laminae 1- 5 in the dorsal horn (Fig. 4G). There were no alterations in total neuron number in the dorsal horn in either WT or VEGFR2scECKO mice (supplemental Figure 4B and C, available at https://links.lww.com/PAIN/B597). VEGFR2scECKO mice had heat hypersensitivity (Fig. 4H) but no alterations in mechanical withdrawal thresholds (Fig. 4I) or motor behaviour (supplemental Figure 4D, available at https://links.lww.com/PAIN/B597). No change in heat withdrawal latency or mechanical withdrawal threshold was identified in WT mice after OHT treatment (Figs. 4H and I).

F4Figure 4.:

Spinal cord VEGFR2 knockout induced vasculopathy causes hypoxia and chronic pain. (A) VEGFR2scECKO mice have degeneration of the dorsal horn endothelium 8 days after intrathecal injection of hydroxytamoxifen (OHT), highlighted by the representative confocal images of the CD31-labelled endothelium (scale bar = 50 μm). OHT treatment led to (B) reduced vessel diameter and (C) decreased vessel volume in VEGFR2scECKO mice (*P < 0.05, ***P < 0.001, unpaired t test, n= 4 per group). (D) TRITC dextran was intravenously injected into terminally anaesthetized WT and VEGFR2scECKO mice and TRITC Dextran-positive perfused vessels identified as outlined in the representative confocal images (scale bar = 50 μm). (E) In VEGFR2scECKO mice, there was less TRITC Dextran in the dorsal horn compared with WT mice (**P < 0.01, unpaired t test, n= 5 per group). (F) Representative confocal images of hypoxyprobe-labelled neurons (colabelled with NeuN) in the dorsal horn of WT and VEGFR2scECKO mice (high-power images = 50 μm). (G) There was an increased percentage of neurons positive for hypoxyprobe, in each laminae 1- 5 across the dorsal horn in VEGFR2scECKO mice (***P < 0.001 2-way ANOVA with Tukey multiple comparison, n = 5 per group). (H) VEGFR2scECKO mice developed reduced withdrawal latencies to heat (***P < 0.001 2-way ANOVA with Tukey multiple comparison, n = 16 per experimental group). (I) No change to mechanical withdrawal threshold was observed (n = 16 per experimental group). TRITC, tetramethylrhodamine isothiocyanate; VEGF, vascular endothelial growth factor; VEGFR2, VEGF receptor 2.

3.3. Induction of hypoxia signalling in the dorsal horn neurons

VEGFR2scECKO mice demonstrated an increase in expression of hypoxia-inducible factor 1α (HIF1α) and glucose transporter 3 (GLUT3, supplemental Figure 5A and B, available at https://links.lww.com/PAIN/B597) in dorsal horn neurons (colabelled with neuronal marker NeuN, Fig. 5 A and 5B; supplemental Figure 5C and D, available at https://links.lww.com/PAIN/B597) and a sig

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