Botts SR, Fish JE, Howe KL. Dysfunctional vascular endothelium as a driver of atherosclerosis: emerging insights into pathogenesis and treatment. Front Pharmacol. 2021;12: 787541.

CAS  PubMed  PubMed Central  Google Scholar 

Falcinelli E, Francisci D, Belfiori B, Petito E, Guglielmini G, Malincarne L, et al. In vivo platelet activation and platelet hyperreactivity in abacavir-treated HIV-infected patients. Thromb Haemost. 2013;110:349–57.

CAS  PubMed  Google Scholar 

Falcinelli E, Cosmi B, Filippini M, Petito E, Legnani C, Cini M, et al. Endothelial activation in patients with superficial vein thrombosis (SVT) of the lower limbs. Thromb Res. 2017;157:20–2.

CAS  PubMed  Google Scholar 

Falcinelli E, Petito E, Becattini C, De Robertis E, Paliani U, Sebastiano M, et al. Role of endothelial dysfunction in the thrombotic complications of COVID-19 patients. J Infect. 2021;82:186–230.

CAS  PubMed  Google Scholar 

Francisci D, Falcinelli E, Belfiori B, Petito E, Fierro T, Baldelli F, et al. Impact of tenofovir versus abacavir on HIV-related endothelial dysfunction. AIDS Patient Care STDs. 2011;25:567–9.

PubMed  Google Scholar 

Francisci D, Falcinelli E, Baroncelli S, Petito E, Cecchini E, Weimer LE, et al. Potential anti-inflammatory effects of maraviroc in HIV-positive patients: a pilot study of inflammation, endothelial dysfunction, and coagulation markers. Scand J Infect Dis. 2014;46:466–70.

CAS  PubMed  Google Scholar 

Gresele P, Migliacci R, Vedovati MC, Ruffatti A, Becattini C, Facco M, et al. Patients with primary antiphospholipid antibody syndrome and without associated vascular risk factors present a normal endothelial function. Thromb Res. 2009;123:444–51.

CAS  PubMed  Google Scholar 

Hedar AM, Stradner MH, Roessler A, Goswami N. Autoimmune rheumatic diseases and vascular function: the concept of autoimmune atherosclerosis. J Clin Med. 2021;10:4427.

CAS  PubMed  PubMed Central  Google Scholar 

Lauper K, Courvoisier DS, Chevallier P, Finckh A, Gabay C. Incidence and prevalence of major adverse cardiovascular events in rheumatoid arthritis, psoriatic arthritis, and axial spondyloarthritis. Arthritis Care Res. 2018;70:1756–63.

Google Scholar 

Giannini S, Falcinelli E, Bury L, Guglielmini G, Rossi R, Momi S, et al. Interaction with damaged vessel wall in vivo in humans induces platelets to express CD40L resulting in endothelial activation with no effect of aspirin intake. Am J Physiol Heart Circ Physiol. 2011;300:H2072-2079.

CAS  PubMed  Google Scholar 

André P, Nannizzi-Alaimo L, Prasad SK, Phillips DR. Platelet-derived CD40L: the switch-hitting player of cardiovascular disease. Circulation. 2002;106:896–9.

PubMed  Google Scholar 

Cognasse F, Duchez AC, Audoux E, Ebermeyer T, Arthaud CA, Prier A, et al. Platelets as key factors in inflammation: focus on CD40L/CD40. Front Immunol. 2022;13: 825892.

CAS  PubMed  PubMed Central  Google Scholar 

Lievens D, Zernecke A, Seijkens T, Soehnlein O, Beckers L, Munnix ICA, et al. Platelet CD40L mediates thrombotic and inflammatory processes in atherosclerosis. Blood. 2010;116:4317–27.

CAS  PubMed  PubMed Central  Google Scholar 

Bartoloni E, Alunno A, Bistoni O, Caterbi S, Luccioli F, Santoboni G, et al. Characterization of circulating endothelial microparticles and endothelial progenitor cells in primary Sjögren’s syndrome: new markers of chronic endothelial damage? Rheumatol Oxf Engl. 2015;54:536–44.

CAS  Google Scholar 

Farinacci M, Krahn T, Dinh W, Volk H-D, Düngen H-D, Wagner J, et al. Circulating endothelial cells as biomarker for cardiovascular diseases. Res Pract Thromb Haemost. 2019;3:49–58.

CAS  PubMed  Google Scholar 

Hur J, Yang H-M, Yoon C-H, Lee C-S, Park K-W, Kim J-H, et al. Identification of a novel role of T cells in postnatal vasculogenesis: characterization of endothelial progenitor cell colonies. Circulation. 2007;116:1671–82.

PubMed  Google Scholar 

Alunno A, Ibba-Manneschi L, Bistoni O, Cipriani S, Topini F, Gerli R, et al. Angiogenic T cells in primary Sjögren’s syndrome: a double-edged sword? Clin Exp Rheumatol. 2019;37(Suppl 118):36–41.

PubMed  Google Scholar 

López P, Rodríguez-Carrio J, Martínez-Zapico A, Caminal-Montero L, Suarez A. Senescent profile of angiogenic T cells from systemic lupus erythematosus patients. J Leukoc Biol. 2016;99:405–12.

PubMed  Google Scholar 

Miao J, Qiu F, Li T, Zhao P, Zhang K, Lv M, et al. Circulating angiogenic T cells and their subpopulations in patients with systemic lupus erythematosus. Mediators Inflamm. 2016;2016:2842143.

PubMed  PubMed Central  Google Scholar 

Rodríguez-Carrio J, Alperi-López M, López P, Alonso-Castro S, Ballina-García FJ, Suárez A. Angiogenic T cells are decreased in rheumatoid arthritis patients. Ann Rheum Dis. 2015;74:921–7.

PubMed  Google Scholar 

de Boer SA, Reijrink M, Abdulahad WH, Hoekstra ES, Slart RHJA, Heerspink HJL, et al. Angiogenic T cells are decreased in people with type 2 diabetes mellitus and recruited by the dipeptidyl peptidase-4 inhibitor Linagliptin: a subanalysis from a randomized, placebo-controlled trial (RELEASE study). Diabetes Obes Metab. 2020;22:1220–5.

PubMed  PubMed Central  Google Scholar 

Zhang G, Liu Y, Qiu Y, Zhang J, Sun J, Zhou Z, et al. Circulating senescent angiogenic T cells are linked with endothelial dysfunction and systemic inflammation in hypertension. J Hypertens. 2021;39:970–8.

CAS  PubMed  Google Scholar 

Bălănescu A-R, Bojincă VC, Bojincă M, Donisan T, Bălănescu SM. Cardiovascular effects of methotrexate in immune-mediated inflammatory diseases. Exp Ther Med. 2019;17:1024–9.

PubMed  Google Scholar 

Johnson TM, Sayles HR, Baker JF, George MD, Roul P, Zheng C, et al. Investigating changes in disease activity as a mediator of cardiovascular risk reduction with methotrexate use in rheumatoid arthritis. Ann Rheum Dis. 2021;80:1385–92.

CAS  PubMed  Google Scholar 

Roubille C, Richer V, Starnino T, McCourt C, McFarlane A, Fleming P, et al. The effects of tumour necrosis factor inhibitors, methotrexate, non-steroidal anti-inflammatory drugs and corticosteroids on cardiovascular events in rheumatoid arthritis, psoriasis and psoriatic arthritis: a systematic review and meta-analysis. Ann Rheum Dis. 2015;74:480–9.

CAS  PubMed  Google Scholar 

Deyab G, Hokstad I, Whist JE, Smastuen MC, Agewall S, Lyberg T, et al. Methotrexate and anti-tumor necrosis factor treatment improves endothelial function in patients with inflammatory arthritis. Arthritis Res Ther. 2017;19:232.

PubMed  PubMed Central  Google Scholar 

Verhoeven F, Prati C, Chouk M, Demougeot C, Wendling D. Methotrexate and cardiovascular risk in rheumatic diseases: a comprehensive review. Expert Rev Clin Pharmacol. 2021;14:1105–12.

CAS  PubMed  Google Scholar 

Liu D, Lv H, Liu Q, Sun Y, Hou S, Zhang L, et al. Atheroprotective effects of methotrexate via the inhibition of YAP/TAZ under disturbed flow. J Transl Med. 2019;17:378.

CAS  PubMed  PubMed Central  Google Scholar 

Ma Y, Li L, Shao Y, Bai X, Bai T, Huang X. Methotrexate improves perivascular adipose tissue/endothelial dysfunction via activation of AMPK/eNOS pathway. Mol Med Rep. 2017;15:2353–9.

CAS  PubMed  Google Scholar 

Thornton CC, Al-Rashed F, Calay D, Birdsey GM, Bauer A, Mylroie H, et al. Methotrexate-mediated activation of an AMPK-CREB-dependent pathway: a novel mechanism for vascular protection in chronic systemic inflammation. Ann Rheum Dis. 2016;75:439–48.

CAS  PubMed  Google Scholar 

Yang D, Haemmig S, Zhou H, Pérez-Cremades D, Sun X, Chen L, et al. Methotrexate attenuates vascular inflammation through an adenosine-microRNA-dependent pathway. eLife. 2021;10:e58064.

CAS  PubMed  PubMed Central  Google Scholar 

Zălar D-M, Pop C, Buzdugan E, Kiss B, Ştefan M-G, Ghibu S, et al. Pharmacological effects of methotrexate and infliximab in a rats model of diet-induced dyslipidemia and beta-3 overexpression on endothelial cells. J Clin Med. 2021;10:3143.

PubMed  PubMed Central  Google Scholar 

Deyab G, Reine TM, Vuong TT, Jenssen T, Hjeltnes G, Agewall S, et al. Antirheumatic treatment is associated with reduced serum Syndecan-1 in rheumatoid arthritis. PLoS ONE. 2021;16: e0253247.

CAS  PubMed  PubMed Central  Google Scholar 

Aletaha D, Neogi T, Silman AJ, Funovits J, Felson DT, Bingham CO, et al. 2010 Rheumatoid arthritis classification criteria: an American College of Rheumatology/European League Against Rheumatism collaborative initiative. Arthritis Rheum. 2010;62:2569–81.

PubMed  Google Scholar 

Mach F, Baigent C, Catapano AL, Koskinas KC, Casula M, Badimon L, et al. 2019 ESC/EAS Guidelines for the management of dyslipidaemias: lipid modification to reduce cardiovascular risk. Eur Heart J. 2020;41:111–88.

PubMed  Google Scholar 

Collins GS, Altman DG. Predicting the 10 year risk of cardiovascular disease in the United Kingdom: independent and external validation of an updated version of QRISK2. BMJ. 2012;344: e4181.

PubMed  PubMed Central  Google Scholar 

Gresele P, Migliacci R, Procacci A, De Monte P, Bonizzoni E. Prevention by NCX 4016, a nitric oxide-donating aspirin, but not by aspirin, of the acute endothelial dysfunction induced by exercise in patients with intermittent claudication. Thromb Haemost. 2007;97:444–50.

CAS  PubMed  Google Scholar 

Gerli R, Schillaci G, Giordano A, Bocci EB, Bistoni O, Vaudo G, et al. CD4+CD28- T lymphocytes contribute to early atherosclerotic damage in rheumatoid arthritis patients. Circulation. 2004;109:2744–8.

CAS  PubMed  Google Scholar 

Barbati C, Vomero M, Colasanti T, Ceccarelli F, Marcosano M, Miranda F, et al. Microparticles and autophagy: a new frontier in the understanding of atherosclerosis in rheumatoid arthritis. Immunol Res. 2018;66:655–62.

CAS  PubMed  Google Scholar 

van Zonneveld AJ, de Boer HC, van der Veer EP, Rabelink TJ. Inflammation, vascular injury and repair in rheumatoid arthritis. Ann Rheum Dis. 2010;69(Suppl 1):i57-60.

PubMed  Google Scholar 

Westerweel PE, Verhaar MC. Endothelial progenitor cell dysfunction in rheumatic disease. Nat Rev Rheumatol. 2009;5:332–40.

CAS  PubMed  Google Scholar 

Mangoni AA, Zinellu A, Sotgia S, Carru C, Piga M, Erre GL. Protective effects of methotrexate against proatherosclerotic cytokines: a review of the evidence. Mediators Inflamm. 2017;2017:9632846.

PubMed  PubMed Central