Wei, C. M., Gershowitz, A. & Moss, B. Methylated nucleotides block 5′ terminus of HeLa cell messenger RNA. Cell 4, 379–386 (1975).
Sommer, S., Lavi, U. & Darnell, J. E. Jr. The absolute frequency of labeled N-6-methyladenosine in HeLa cell messenger RNA decreases with label time. J. Mol. Biol. 124, 487–499 (1978).
Meyer, K. D. et al. Comprehensive analysis of mRNA methylation reveals enrichment in 3′ UTRs and near stop codons. Cell 149, 1635–1646 (2012).
CAS PubMed PubMed Central Google Scholar
Dominissini, D. et al. Topology of the human and mouse m6A RNA methylomes revealed by m6A-seq. Nature 485, 201–206 (2012). Together with Meyer et al. (2012), this paper reports the mapping of m6A in the human and mouse transcriptome.
Tegowski, M., Flamand, M. N. & Meyer, K. D. scDART-seq reveals distinct m6A signatures and mRNA methylation heterogeneity in single cells. Mol. Cell 82, 868–878.e10 (2022).
Liu, J. et al. A METTL3–METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation. Nat. Chem. Biol. 10, 93–95 (2014). This paper reports that the METTL3–METTL14–WTAP complex mediates N6-adenosine methylation.
Wei, C. M. & Moss, B. Nucleotide sequences at the N6-methyladenosine sites of HeLa cell messenger ribonucleic acid. Biochemistry 16, 1672–1676 (1977).
Linder, B. et al. Single-nucleotide-resolution mapping of m6A and m6Am throughout the transcriptome. Nat. Methods 12, 767–772 (2015).
CAS PubMed PubMed Central Google Scholar
Zheng, G. et al. ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility. Mol. Cell 49, 18–29 (2013). This study identifies and characterizes ALKBH5 as an m6A demethylase, demonstrating that m6A is a dynamic reversible modification in mRNA.
Jia, G. et al. N6-Methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO. Nat. Chem. Biol. 7, 885–887 (2011). This study identifies and characterizes FTO as an m6A demethylase, suggesting that m6A is a dynamic reversible modification in mRNA; it should be read in conjunction with Mauer et al. (2017), which suggests that FTO is an m6Am demethylase.
CAS PubMed PubMed Central Google Scholar
Piekna-Przybylska, D., Decatur, W. A. & Fournier, M. J. The 3D rRNA modification maps database: with interactive tools for ribosome analysis. Nucleic Acids Res. 36, D178–D183 (2008).
Sergiev, P. V., Aleksashin, N. A., Chugunova, A. A., Polikanov, Y. S. & Dontsova, O. A. Structural and evolutionary insights into ribosomal RNA methylation. Nat. Chem. Biol. 14, 226–235 (2018).
Ma, H. et al. N6-Methyladenosine methyltransferase ZCCHC4 mediates ribosomal RNA methylation. Nat. Chem. Biol. 15, 88–94 (2019).
Ren, W. et al. Structure and regulation of ZCCHC4 in m6A-methylation of 28S rRNA. Nat. Commun. 10, 5042 (2019).
PubMed PubMed Central Google Scholar
Pinto, R. et al. The human methyltransferase ZCCHC4 catalyses N6-methyladenosine modification of 28S ribosomal RNA. Nucleic Acids Res. 48, 830–846 (2020).
van Tran, N. et al. The human 18S rRNA m6A methyltransferase METTL5 is stabilized by TRMT112. Nucleic Acids Res. 47, 7719–7733 (2019).
PubMed PubMed Central Google Scholar
Liberman, N. et al. N6-Adenosine methylation of ribosomal RNA affects lipid oxidation and stress resistance. Sci. Adv. 6, eaaz4370 (2020).
CAS PubMed PubMed Central Google Scholar
Xing, M. et al. The 18S rRNA m6A methyltransferase METTL5 promotes mouse embryonic stem cell differentiation. EMBO Rep. 21, e49863 (2020).
CAS PubMed PubMed Central Google Scholar
Rong, B. et al. Ribosome 18S m6A methyltransferase METTL5 promotes translation initiation and breast cancer cell growth. Cell Rep. 33, 108544 (2020).
Leismann, J. et al. The 18S ribosomal RNA m6A methyltransferase Mettl5 is required for normal walking behavior in Drosophila. EMBO Rep. 21, e49443 (2020).
CAS PubMed PubMed Central Google Scholar
Ignatova, V. V. et al. The rRNA m6A methyltransferase METTL5 is involved in pluripotency and developmental programs. Genes Dev. 34, 715–729 (2020).
CAS PubMed PubMed Central Google Scholar
Sendinc, E., Valle-Garcia, D., Jiao, A. & Shi, Y. Analysis of m6A RNA methylation in Caenorhabditis elegans. Cell Discov. 6, 47 (2020).
CAS PubMed PubMed Central Google Scholar
Sepich-Poore, C. et al. The METTL5–TRMT112 N6-methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation. J. Biol. Chem. 298, 101590 (2022).
CAS PubMed PubMed Central Google Scholar
Kierzek, E. & Kierzek, R. The thermodynamic stability of RNA duplexes and hairpins containing N6-alkyladenosines and 2-methylthio-N6-alkyladenosines. Nucleic Acids Res. 31, 4472–4480 (2003).
CAS PubMed PubMed Central Google Scholar
Meyer, K. D. & Jaffrey, S. R. The dynamic epitranscriptome: N6-methyladenosine and gene expression control. Nat. Rev. 15, 313–326 (2014).
Liu, N. et al. N6-Methyladenosine-dependent RNA structural switches regulate RNA–protein interactions. Nature 518, 560–564 (2015). This paper describes a mechanism by which m6A affects RNA folding to indirectly have an impact on the binding of proteins to RNA.
CAS PubMed PubMed Central Google Scholar
Wang, S. et al. The m6A methylation perturbs the Hoogsteen pairing-guided incorporation of an oxidized nucleotide. Chem. Sci. 8, 6380–6388 (2017).
CAS PubMed PubMed Central Google Scholar
Ashraf, S., Huang, L. & Lilley, D. M. J. Effect of methylation of adenine N6 on kink turn structure depends on location. RNA Biol. 16, 1377–1385 (2019).
PubMed PubMed Central Google Scholar
Ogawa, A. et al. N6-Methyladenosine (m6A) is an endogenous A3 adenosine receptor ligand. Mol. Cell 81, 659–674.e7 (2021).
Patil, D. P., Pickering, B. F. & Jaffrey, S. R. Reading m6A in the transcriptome: m6A-binding proteins. Trends Cell Biol. 28, 113–127 (2018).
Shi, H., Wei, J. & He, C. Where, when, and how: context-dependent functions of RNA methylation writers, readers, and erasers. Mol. Cell 74, 640–650 (2019).
CAS PubMed PubMed Central Google Scholar
Zaccara, S., Ries, R. J. & Jaffrey, S. R. Reading, writing and erasing mRNA methylation. Nat. Rev. 20, 608–624 (2019).
Huang, H. et al. Recognition of RNA N6-methyladenosine by IGF2BP proteins enhances mRNA stability and translation. Nat. Cell Biol. 20, 285–295 (2018).
CAS PubMed PubMed Central Google Scholar
Edupuganti, R. R. et al. N6-Methyladenosine (m6A) recruits and repels proteins to regulate mRNA homeostasis. Nat. Struct. Mol. Biol. 24, 870–878 (2017).
CAS PubMed PubMed Central Google Scholar
Ping, X. L. et al. Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase. Cell Res. 24, 177–189 (2014).
CAS PubMed PubMed Central Google Scholar
Fustin, J. M. et al. RNA-methylation-dependent RNA processing controls the speed of the circadian clock. Cell 155, 793–806 (2013).
Meyer, K. D. et al. 5′ UTR m6A promotes cap-independent translation. Cell 163, 999–1010 (2015).
CAS PubMed PubMed Central Google Scholar
Wang, X. et al. N6-Methyladenosine modulates messenger RNA translation efficiency. Cell 161, 1388–1399 (2015). Together with Meyer et al. (2015), this paper establishes a role for m6A in regulating mRNA translation.
CAS PubMed PubMed Central Google Scholar
Zhou, J. et al. Dynamic m6A mRNA methylation directs translational control of heat shock response. Nature 526, 591–594 (2015).
CAS PubMed PubMed Central Google Scholar
Li, A. et al. Cytoplasmic m6A reader YTHDF3 promotes mRNA translation. Cell Res. 27, 444–447 (2017).
CAS PubMed PubMed Central Google Scholar
Wang, X. et al. N6-Methyladenosine-dependent regulation of messenger RNA stability. Nature 505, 117–120 (2014). This paper establishes a role for m6A in modulating mRNA stability.
Viegas, I. J. et al. N6-Methyladenosine in poly(A) tails stabilize VSG transcripts. Nature 604, 362–370 (2022).
Article PubMed PubMed Central Google Scholar
Alarcon, C. R., Lee, H., Goodarzi, H., Halberg, N. & Tavazoie, S. F. N6-Methyladenosine marks primary microRNAs for processing. Nature 519, 482–485 (2015). This paper establishes a role for m6A in modulating miRNA processing.
留言 (0)