Available online 6 October 2022
AbstractBackgroundPalmitoleic acid is a fatty acid that possesses nutritional, health and industrial applications. However, it accumulates in the seed oil of few plant species that often lack agronomic value. A bioinformatics approach was developed as a complementary tool to effort- and time-consuming traditional methods to identify palmitoleic acid accumulating plant species. It consisted in identifying acyl-ACP desaturases with a sequence variation linked to a switch in the substrate preference from stearic to palmitic acid.
ResultsA PHI-BLAST analysis identified Handroanthus impetiginosus as a candidate species with two acyl-ACP desaturases with the desired sequence variation. A substrate docking analysis showed that the presence of phenylalanine at the bottom of the active site plays a similar structural role to that of tryptophan present in the same position in the divergent desaturase of the palmitoleic acid accumulator Dolichandra unguis-cati. The analysis of the genome of H. impetiginosus allowed the identification of four putative ferredoxins, three of which of the heterotrophic type that have been linked to an increase in the activity of unusual acyl-ACP desaturases. RT-PCR results showed that both studied H. impetiginosus desaturases are expressed in pod but not in seeds, while all 4 ferredoxins are expressed in both tissues. GC-MS analysis confirmed the presence of palmitoleic acid in seed oil.
ConclusionsThese results suggest that the proposed bioinformatic approach can be a valuable compliment to traditional methods for identification of plant species that accumulate palmitoleic acid. However, further improvements are needed such as predicting seed expression of desaturases.
KeywordsAccumulation
Acyl-ACP desaturase
Bioinformatic approach
Docking modeling
Fatty acid
Handroanthus impetiginosus
Palmitoleic acid
PHI-BLAST
Plants
Seed oil
© 2022 Published by Elsevier B.V. on behalf of Pontificia Universidad Católica de Valparaíso.
留言 (0)