Available online 27 September 2022, 104737

We developed a novel method to measure 17 nucleosides and creatinine including 5 isomer pairs in 8 min with high sensitivity and high accuracy.

Rapid sample pretreatment was applied to eliminate matrix effect by proper dilution.

The levels of m3C and m1A had significantly change in both CCS and urine, which could be used for non-invasive diagnosis of bladder cancer.

The combination of m3C, m1A, m1G and m22G were identified as the biomarkers for recurrence monitoring of bladder cancer.

The comprehensive results of the nucleosides in CCS and urine would provide more precise results for the diagnosis and prognosis of bladder cancer.

The exploration of nucleoside changes in human biofluids has profound potential for cancer diagnosis. Herein, we developed a rapid methodology to quantify 17 nucleosides by UHPLC-MS/MS. Five pairs of isomers were successfully separated within 8 min. The ME was mostly eliminated by sample dilution folds of 1000 for urine and 40 for CCS. The optimized method was firstly applied to screen potential nucleoside biomarkers in CCS by comprising bladder cancer cell lines (5637 and T24) and normal human bladder cell line SV-HUC-1 together with student's t-test and OPLS-DA. Nucleosides with significant differences in the supernatant of urine samples were also uncovered comparing BCa with the non-tumor group, as well as a comparison of BCa recurrence group with the non-recurrence group. By intersecting the differential nucleosides in CCS and urine supernatant, and then further confirmed using validation sets, the combination of m3C and m1A with AUC of 0.775 was considered as a potential biomarker for bladder cancer diagnosis. A panel of m3C, m1A, m1G, and m22G was defined as potential biomarkers for bladder cancer prognosis with an AUC of 0.819. Above all, this method provided a new perspective for diagnosis and recurrence monitoring of bladder cancer.

The exploration of nucleoside changes in body fluids has profound potential for the diagnosis and elucidation of the pathogenesis of cancer. In this study, we developed a rapid methodology for the simultaneous quantitative determination of 17 nucleosides in the supernatant of cells and urine samples using UHPLC-MS/MS to discover and validate bladder cancer related excreted nucleoside biomarkers. The results of this paper provide a new strategy for diagnosis and postoperative recurrence monitoring of bladder cancer and provide theoretical support for the exploration of its pathogenesis.

Nucleosides

LC-MRM

Matrix effects

Biomarker

Bladder cancer

Cell culture supernatants and urine

ultra-high-performance liquid chromatography-electrospray tandem mass spectrometry

cell culture supernatant

orthogonal partial least squares-discriminant

The area under the curve

© 2022 Published by Elsevier B.V.