Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/60573

metadata.dc.identifier.doi: https://doi.org/10.56042/ijeb.v60i10.41435Title: Escherichia coli biofilm formation and control by phenolic compounds from Salvia officinalis L. extractsAuthors: Stefanović, Olgica
Mladenović, Dijana
Mladenović, Katarina
Grujović, Mirjana
Ivanović, DraganaKeywords: Antimicrobials;Antibiofilm activity;Culinary sage;Garden sageIssue Date: Sep-2022Publisher: NIScPR-CSIR, IndiaAbstract: Biofilm is recognized as one of the virulence factors of uropathogenic Escherichia coli. It offers bacteria significantly increased tolerance to antibiotics and makes difficulties in infection eradication. In this study, the biofilm formation ability of clinical isolates of uropathogenic E. coli as well as the antibiofilm activity of the common Garden sage, Salvia officinalis L.extracts were investigated in vitro. The influence of growth conditions (nutrient composition and incubation period) onbiofilm formation of E. coli strains was evaluated using crystal violet staining procedure. The tested strains better formedbiofilms after a longer incubation period (48 h). In addition, biofilm formation depended on nutrient medium composition.The eight strains were slime producers (Congo red agar assay). The water, ethanol, acetone and diethyl ether S. officinalisextracts were screened for their ability to inhibit the formation and metabolic activity of E. coli biofilms using crystalviolet and resazurin assay, respectively. The water extract was not active, while ethanol, acetone and diethyl etherextract exhibited antibiofilm activity (MBIC= 2.5-10 mg/mL). The microscopic visualization of treated E. coli LM1 biofilmhas shown morphological and density changes. In addition, the concentrations of phenolic compounds were determinedspectrophotometrically. The highest content of total phenolics was found in acetone extract while the flavonoids inwater extract.Page(s): 771-780ISSN: 0975-1009 (Online); 0019-5189 (Print)Appears in Collections:IJEB Vol.60(10) [Oct 2022]

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