Irisin promotes fracture healing by improving osteogenesis and angiogenesis

Journal of Orthopaedic TranslationVolume 37, November 2022, Pages 37-45Journal of Orthopaedic TranslationAbstractBackground

Osteogenesis and angiogenesis are important for bone fracture healing. Irisin is a muscle-derived monokine that is associated with bone formation.

Methods

To demonstrate the effect of irisin on bone fracture healing, closed mid-diaphyseal femur fractures were produced in 8-week-old C57BL/6 mice. Irisin was administrated intraperitoneally every other day after surgery, fracture healing was assessed by using X-rays. Bone morphometry of the fracture callus were assessed by using micro-computed tomography. Femurs of mice from each group were assessed by the three-point bending testing. Effect of irisin on osteogenic differentiation in mesenchymal stem cells in vitro was evaluated by quantitative real-time polymerase chain reaction (qRT-PCR), alkaline phosphatase staining and alizarin red staining. Angiogenesis of human umbilical vein endothelial cells (HUVECs) were evaluated by qRT-PCR, migration tests, and tube formation assays.

Results

Increased callus formation, mineralization and tougher fracture healing were observed in the irisin-treated group than in the control group, indicating the better fracture callus healing due to Irisin treatment. The vessel surface and vessel volume fraction of the callus also increased in the irisin-treated group. The expression of BMP2, CD31, and VEGF in callus were enhanced in the irisin-treated group. In mouse bone mesenchymal stem cells, irisin promoted ALP expression and mineralization, and increased the expression of osteogenic genes, including OSX, Runx2, OPG, ALP, OCN and BMP2. Irisin also promoted HUVEC migration and tube formation. Expression of angiogenic genes, including ANGPT1, ANGPT2, VEGFb, CD31, FGF2, and PDGFRB in HUVECs were increased by irisin.

Conclusion

All the results indicate irisin can promote fracture healing through osteogenesis and angiogenesis. These findings help in the understanding of muscle–bone interactions during fracture healing.

The Translational Potential of this Article

Irisin was one of the most important monokine secreted by skeletal muscle. Studies have found that irisin have anabolic effect one bone remodeling through affecting osteocyte and osteoblast. Based on our study, irisin could promote bone fracture healing by increasing bone mass and vascularization, which provide a potential usage of irisin to promote fracture healing and improve clinical outcomes.

Keywords

Fracture healing

Myokine

Irisin

Osteogenesis

Angiogenesis

AbbreviationsBMP2

bone morphogenetic protein 2

CD31

platelet endothelial cell adhesion molecule-1

VEGF

vascular endothelial growth factor

Runx2

core-binding factor, alpha 3 subunit

HUVEC

human umbilical vein endothelial cells

VEGFb

vascular endothelial growth factor b

FGF2

fibroblast growth factor 2

PDGFRB

platelet-derived growth factor receptor b

© 2022 The Authors. Published by Elsevier B.V. on behalf of Chinese Speaking Orthopaedic Society.

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