An Optimised Monophasic Faecal Extraction Method for LC-MS Analysis and its Application in gastrointestinal Disease

Abstract

Liquid chromatography coupled with mass spectrometry (LC-MS) metabolomic approaches are widely used to investigate underlying pathogenesis of gastrointestinal disease and mechanism of action of treatments. However, a standardised method for extracting metabolites from faecal samples for large-scale metabolomic studies is yet to be defined. Current methods often rely on biphasic extractions using harmful halogenated solvents, making automation and large-scale studies challenging. The present study reports an optimised monophasic faecal extraction protocol that is suitable for untargeted and targeted LC-MS analyses. The impact of several experimental parameters, including sample weight, extraction solvent, cellular disruption method, and sample-to-solvent ratio were investigated. It is suggested that a 50 mg freeze-dried faecal sample should be used in a methanol extraction (1:20) using bead beating as the means of cell disruption. This is revealed by a significant increase in number of metabolites detected, improved signal intensity, and wide metabolic coverage given by each of the above extraction parameters. Finally, we addressed the applicability of the method on faecal samples from patients with Crohns disease (CD) and coeliac disease (CoD), two distinct chronic gastrointestinal diseases involving metabolic perturbations. Untargeted and targeted metabolomic analysis demonstrated the ability of the developed method to detect and stratify metabolites extracted from patient groups and healthy controls (HC), highlighting characteristic changes in the faecal metabolome according to disease. The method developed is therefore suitable for the analysis of patients with gastrointestinal disease and can be used to detect and distinguish differences in the metabolomes of CD, CoD, and HC.

Competing Interest Statement

The authors have declared no competing interest.

Funding Statement

This study was supported by Shimadzu UK and the University of Strathclyde through joint contribution to P.E.Ks studentship. The clinical studies were funded by the Glasgow Children Hospital Charity and the Nutricia Research Foundation. ZR would like to acknowledge the EPSRC Multiscale Metrology Suite (EP/V028960/1).

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I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained.

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The details of the IRB/oversight body that provided approval or exemption for the research described are given below:

All participants and their carers provided written informed consent. The study was approved by the NHS West of Scotland Research Ethics Committee (Ref: 11/WS/0006) for the study in patients with coeliac disease and the Yorkhill Research Ethics Committee (Reb: 05/S0708/66) for the study in patients with Crohns disease.

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Data Availability

All data produced in the present study are available upon reasonable request to the authors.

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