Sinkora, M., J. Sinkorova, and K. Stepanova. 2017. Ig light chain precedes heavy chain gene rearrangement during development of B cells in swine. J. Immunol. 198: 1543–1552.

There was an error in the legend for Fig. 2 as originally published. The mentions of CD179a in the legend should have been mentions of CD172a. The corrected version of the figure legend is shown below.

FIGURE 2. Analysis of IgL expression on different subpopulations of porcine cells. Fresh BM cells isolated from newborn pigs were gated for large (A–D) and small (E–H) MHC-II+ cells only, and their representative surface expression of CD172a and either IgHμ or IgLλ detected by FCM on the surface (A, B, E, and F) or intracellularly (C, D, G, and H) are shown. Staining for IgLκ (data not shown) gave a similar result as for IgLλ. Filled arrows indicate large MHC-II+CD172ahiIgLλ+IgH− early precursors whereas open arrows indicate small MHC-II+CD172a−IgLλ+IgH+ immature B cells. Results are representative of nine independent experiments using fetal, young, and adult pigs. Comparison of IgHμ and IgLλ surface expression on BM (I) and spleen (J) cells is also shown. Western blot under reducing conditions (K, left) demonstrates that IgH is absent in sorted large MHC-II+CD172ahiIgLλ+IgH− early precursors (filled arrow) whereas it is present in small MHC-II+CD172a−IgLλ+IgH+ immature B cells (open arrow). Shown is also a confirmation that anti-porcine IgHμ mAbs (M145 and M160) recognize IgHμ from porcine serum and cells (∼75 kDa), which is different from other Ig classes (∼50–60 kDa). Secondary peroxidase-conjugated horse anti-mouse Abs do not recognize swine Igs. Note that mouse IgG (moIgG) and IgM (moIgM) were used for comparison, and moIgM was detected differently by GoαMo-IgM followed by MoαGo-HRP Abs (indicated by asterisk). The dashed vertical lines between gel strips indicate where parts of the images were jointed from different gels. A loading control stained by Coomassie blue is also shown (K, right).