Research investigating how the brain develops and learns profoundly impacts education. Understand the brain mechanisms responsible for learning and memory and factors that influence them, such as age, environment, emotions, and motivation, can transform educational strategies by contributing to the development of programs that optimize learning. Including neuroscience education in teachers' training requires teaching them a multidisciplinary approach to science, which presents a challenge. Furthermore, the potential educational advances form the incorporation of neuroscience into teachers' training are hindered by significant obstacles, such as translate research into the classroom; this include the spread of neuromyths and the products, practices, and programs based on them. Our group has nine years of experience in developing courses for training teachers. However, in 2020, the world faced the COVID-19 pandemic, which imposed in society a new way of carrying out its daily activities, including teaching. This study reports the experience of our group as we developed the ninth edition of the Neuroscience Applied to Education teachers' training in the online format that included synchronous and asynchronous activities. Sixty (60) teachers participated in the course. The synchronous meeting lasted ~1.5 hours/week and dressed different themes: neuroscience and education, neuroanatomy, neurophysiology, neurobiology of learning and memory, factors that interfere with learning, and pedagogical innovation. According to the teachers' perception, the course was fundamental for them in terms of acquiring new knowledge about neuroscience. Everyone agreed on the possible applicability of the concepts covered to improve their pedagogical practice and teacher environment.