Available online 16 September 2022, 104612
Highlights•Serum IgA bound to BSMCs and induced production of IL-6 and IL-8.
•Serum IgA enhanced cell proliferation and migration by BSMCs.
•Serum IgA promoted BSMCs phenotypic switch toward synthetic.
•TfR is partially involved in production of cytokines from the BSMCs by serum IgA.
AbstractImmunoglobulin A (IgA) is important in biological defense, mainly in the mucosal area, and plays pathogenic roles in various diseases by activating both inflammatory and structural cells. The current study aimed to validate the effects of IgA on the human bronchial smooth muscle cell (BSMC), which plays a major role in airway inflammation and remodeling. Serum IgA induced interleukin (IL)-6 and IL-8 production at both mRNA and protein levels, and enhanced cell proliferation and migration by the BSMCs. The synthetic phenotype markers were regulated and the contractile phenotype markers were downregulated by serum IgA. Mitogen-activated protein kinase, phosphatidylinositol 3-kinase/Akt, and nuclear factor-κB pathways were involved in IgA-induced IL-6 and IL-8 production. The BSMCs expressed transferrin receptor (TfR), and TfR siRNA transfection inhibited IL-6 and IL-8 production by serum IgA. In summary, serum IgA is a potent activator of the BSMCs at least partially via TfR.
KeywordsBronchial smooth muscle cells
IgA
Human
Inflammation
Phenotypic modulation
Abbreviationsα-SMAα-smooth muscle actin
ASGPRasialoglycoprotein receptor
BSMCbronchial smooth muscle cell
β4GalT1β 1, 4-galactosyltransferase 1
DMEMDulbecco’s Modified Eagle’s Medium
ERKextracellular signal-regulated kinase
FITCfluorescein isothiocyanate
GAPDHglyceraldehyde 3-phosphate dehydrogenase
HB-EGFheparin-binding epidermal growth factor-like growth factor
MAPKmitogen-activated protein kinase
PDGFplatelet-derived growth factor
PI3Kphosphatidylinositol 3-kinase
pIgRpolymeric immunoglobulin receptor
RT-qPCRreal-time quantitative polymerase chain reaction
siRNAsmall interference RNA
SEMstandard error of the mean
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