Available online 13 September 2022
AbstractBackgroundHong Jiang (HC), a grafted chimera of sweet orange (Citrus sinensis (L.) Osbeck), is prone to variations in fruit shape, taste, and pulp mastication. We studied the transcriptomes and metabolomes pf pulps of HC and its two variations (CB: fruits with changed pulp mastication, taste, and color and JB: fruits with changed pulp color and taste) to explore the related pathways.
ResultsJB accumulated higher organic acids as compared to HC and CB. Flavonoid content was highest in HC followed by JB and CB. The soluble sugar content was lower, while cellulose content was higher in both JB and CB as compared to HC. We found 5,156 and 1,673 DEGs and 283 and 94 DAMs in HC vs JB and HC vs CB, respectively. The differential regulation of starch and sucrose metabolism, galactose metabolism, glycolysis/gluconeogenesis, fructose and mannose metabolism, and citrate cycle pathways could be associated with changes in sugar contents and tastes in JB and CB. Cell-wall polymers-related DEGs/DAMs were associated with the inferior mastication quality of JB and CB. Carotenoid biosynthesis possibly imparts yellowish and reddish pulp color in HC. Additional to this pathway, the anthocyanin biosynthesis led to the changes in JB and CB pulp color.
ConclusionsThis combined methodological approach proved to be useful in delineating the large-scale changes in the transcripts and metabolites of variant fruits in a chimeric citrus variety. This study provides advanced and large-scale data on citrus taste, mastication, and pulp color.
KeywordsBud sport mutants
Citrus sinensis
Color
Fruit
Hong Jiang
Mastication
Metabolome
Pulp
Shape
Sweet orange
Taste
Transcriptome
AbbreviationsDEGdifferentially expressed gene
FPKMfragments per kilobase of exon model per million reads mapped
KEGGKyoto Encyclopedia of Genes and Genomes
PCAprincipal component analysis
qRT-PCRquantitative real-time PCR
Data availabilityThe raw RNA-seq data has been submitted to NCBI SRA under the project number: PRJNA739389 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA739389).
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