First near-complete CVA22 sequences from Europe.

Genomic characterization of CVA22 recovered from clinical isolates.

Relative frequency of CVA22 in a regional university hospital.

Prolonged CVA22 infection in a transplant recipient over 5 years.

Enteroviruses are highly diverse with a wide spectrum of genotypes and clinical manifestations. Coxsackievirus A22 (CVA22) has been detected globally from sewage surveillance; however, currently there is limited information on its prevalence in patients, as well as available genomic data.

We aimed to provide genomic and relative frequency data on CVA22 from a regional hospital perspective between 2013–2020.

Sanger sequencing was performed on all samples with a positive enterovirus RT-qPCR result (≤Ct 32). Viral targeted sequence capture (ViroCap) and next-generation sequencing (NGS) (Illumina NextSeq 500) was used to characterize and generate near-complete CVA22 genomes for enteroviruses without genotyping results from Sanger sequencing. Epidemiological and phylogenetic analysis was performed using maximum likelihood trees on MEGA-11.

A total of twenty detections derived from fecal material from sixteen patients were observed between 2013– 2020. One transplant recipient had five different CVA22 infection episodes over five years, with phylogenetic analysis indicating possible reinfection with an additional prolonged infection (>3 weeks). Furthermore, we report the first two near-complete CVA22 sequences from Europe, which grouped with a strain previously isolated from Bangladesh in 1999.

We show a highly diverse enterovirus genotype which causes infections annually, typically in autumn and winter, and is capable of recurrent infection in an immunocompromised patient. Furthermore, we highlight the use of NGS to complement conventional targeted Sanger sequencing.

Enterovirus

Coxsackievirus A22

Next-generation sequencing

Prolonged infection

© 2022 The Authors. Published by Elsevier B.V.