The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination

Knipscheer, P. et al. The Fanconi anemia pathway promotes replication-dependent DNA interstrand cross-link repair. Science 326, 1698–1701 (2009).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Raschle, M. et al. Mechanism of replication-coupled DNA interstrand crosslink repair. Cell 134, 969–980 (2008).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Semlow, D. R. & Walter, J. C. Mechanisms of vertebrate DNA interstrand cross-link repair. Annu. Rev. Biochem. 90, 107–135 (2021).

CAS  PubMed  Article  Google Scholar 

Crossan, G. P. & Patel, K. J. The Fanconi anaemia pathway orchestrates incisions at sites of crosslinked DNA. J. Pathol. 226, 326–337 (2012).

CAS  PubMed  Article  Google Scholar 

Walden, H. & Deans, A. J. The Fanconi anemia DNA repair pathway: structural and functional insights into a complex disorder. Annu. Rev. Biophys. 43, 257–278 (2014).

CAS  PubMed  Article  Google Scholar 

Alpi, A. et al. UBE2T, the Fanconi anemia core complex, and FANCD2 are recruited independently to chromatin: a basis for the regulation of FANCD2 monoubiquitination. Mol. Cell. Biol. 27, 8421–8430 (2007).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Garcia-Higuera, I. et al. Interaction of the Fanconi anemia proteins and BRCA1 in a common pathway. Mol. Cell 7, 249–262 (2001).

CAS  PubMed  Article  Google Scholar 

Machida, Y. J. et al. UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation. Mol. Cell 23, 589–596 (2006).

CAS  PubMed  Article  Google Scholar 

Meetei, A. R. et al. A novel ubiquitin ligase is deficient in Fanconi anemia. Nat. Genet. 35, 165–170 (2003).

CAS  PubMed  Article  Google Scholar 

Smogorzewska, A. et al. Identification of the FANCI protein, a monoubiquitinated FANCD2 paralog required for DNA repair. Cell 129, 289–301 (2007).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Klein Douwel, D. et al. XPF-ERCC1 acts in unhooking DNA interstrand crosslinks in cooperation with FANCD2 and FANCP/SLX4. Mol. Cell 54, 460–471 (2014).

CAS  PubMed  Article  Google Scholar 

Taniguchi, T. et al. S-phase-specific interaction of the Fanconi anemia protein, FANCD2, with BRCA1 and RAD51. Blood 100, 2414–2420 (2002).

CAS  PubMed  Article  Google Scholar 

Kim, J. M. et al. Inactivation of murine Usp1 results in genomic instability and a Fanconi anemia phenotype. Dev. Cell 16, 314–320 (2009).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Nijman, S. M. et al. The deubiquitinating enzyme USP1 regulates the Fanconi anemia pathway. Mol. Cell 17, 331–339 (2005).

CAS  PubMed  Article  Google Scholar 

Oestergaard, V. H. et al. Deubiquitination of FANCD2 is required for DNA crosslink repair. Mol. Cell 28, 798–809 (2007).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Alcon, P. et al. FANCD2-FANCI is a clamp stabilized on DNA by monoubiquitination of FANCD2 during DNA repair. Nat. Struct. Mol. Biol. 27, 240–248 (2020).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Joo, W. et al. Structure of the FANCI-FANCD2 complex: insights into the Fanconi anemia DNA repair pathway. Science 333, 312–316 (2011).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Rennie, M. L., Arkinson, C., Chaugule, V. K., Toth, R. & Walden, H. Structural basis of FANCD2 deubiquitination by USP1-UAF1. Nat. Struct. Mol. Biol. 28, 356–364 (2021).

CAS  PubMed  Article  Google Scholar 

Rennie, M. L. et al. Differential functions of FANCI and FANCD2 ubiquitination stabilize ID2 complex on DNA. EMBO Rep. 21, e50133 (2020).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Shakeel, S. et al. Structure of the Fanconi anaemia monoubiquitin ligase complex. Nature 575, 234–237 (2019).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Tan, W. et al. Monoubiquitination by the human Fanconi anemia core complex clamps FANCI:FANCD2 on DNA in filamentous arrays. eLife 9, e54128 (2020).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Wang, R., Wang, S., Dhar, A., Peralta, C. & Pavletich, N. P. DNA clamp function of the monoubiquitinated Fanconi anaemia ID complex. Nature 580, 278–282 (2020).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Wang, S., Wang, R., Peralta, C., Yaseen, A. & Pavletich, N. P. Structure of the FA core ubiquitin ligase closing the ID clamp on DNA. Nat. Struct. Mol. Biol. 28, 300–309 (2021).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Rajendra, E. et al. The genetic and biochemical basis of FANCD2 monoubiquitination. Mol. Cell 54, 858–869 (2014).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Sato, K., Toda, K., Ishiai, M., Takata, M. & Kurumizaka, H. DNA robustly stimulates FANCD2 monoubiquitylation in the complex with FANCI. Nucleic Acids Res. 40, 4553–4561 (2012).

CAS  PubMed  PubMed Central  Article  Google Scholar 

van Twest, S. et al. Mechanism of ubiquitination and deubiquitination in the Fanconi anemia pathway. Mol. Cell 65, 247–259 (2017).

PubMed  Article  CAS  Google Scholar 

Ishiai, M. et al. FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway. Nat. Struct. Mol. Biol. 15, 1138–1146 (2008).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Andreassen, P. R., D’Andrea, A. D. & Taniguchi, T. ATR couples FANCD2 monoubiquitination to the DNA-damage response. Genes Dev. 18, 1958–1963 (2004).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Cheung, R. S. et al. Ubiquitination-linked phosphorylation of the FANCI S/TQ cluster contributes to activation of the Fanconi anemia I/D2 complex. Cell Rep. 19, 2432–2440 (2017).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Shigechi, T. et al. ATR-ATRIP kinase complex triggers activation of the Fanconi anemia DNA repair pathway. Cancer Res. 72, 1149–1156 (2012).

CAS  PubMed  Article  Google Scholar 

Tan, W., van Twest, S., Murphy, V. J. & Deans, A. J. ATR-mediated FANCI phosphorylation regulates both ubiquitination and deubiquitination of FANCD2. Front. Cell Dev. Biol. 8, 2 (2020).

PubMed  PubMed Central  Article  Google Scholar 

Ishiai, M. et al. Activation of the FA pathway mediated by phosphorylation and ubiquitination. Mutat. Res. 803805, 89–95 (2017).

PubMed  Article  CAS  Google Scholar 

Chaugule, V. K., Arkinson, C., Toth, R. & Walden, H. Enzymatic preparation of monoubiquitinated FANCD2 and FANCI proteins. Methods Enzymol. 618, 73–104 (2019).

CAS  PubMed  Article  Google Scholar 

Russo, C. J. & Passmore, L. A. Electron microscopy: ultrastable gold substrates for electron cryomicroscopy. Science 346, 1377–1380 (2014).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Zivanov, J. et al. New tools for automated high-resolution cryo-EM structure determination in RELION-3. eLife 7, e42166 (2018).

PubMed  PubMed Central  Article  Google Scholar 

Zheng, S. Q. et al. MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Nat. Methods 14, 331–332 (2017).

CAS  PubMed  PubMed Central  Article  Google Scholar 

Rohou, A. & Grigorieff, N. CTFFIND4: fast and accurate defocus estimation from electron micrographs. J. Struct. Biol. 192, 216–221 (2015).

PubMed  PubMed Central  Article 

留言 (0)

沒有登入
gif