Singh N, Bhalla M, De Jager P, Gilca M (2011) An overview on ashwagandha: a rasayana (Rejuvenator) of ayurveda. Afr J Tradit Complement Altern Med 8(5S):208–213. https://doi.org/10.4314/ajtcam.v8i5s.9

Article  PubMed  PubMed Central  Google Scholar 

Tiwari R, Chakraborty S, Saminathan M, Dhama K, Singh SV (2014) Ashwagandha (Withania somnifera): role in safeguarding health, immunomodulatory effects, combating infections and therapeutic applications: A review. J Biol Sci 14(2):77–94. https://doi.org/10.3923/jbs.2014.77.94

CAS  Article  Google Scholar 

Verma N, Gupta SK, Tiwari S, Mishra AK (2021) Safety of ashwagandha root extract: a randomized, placebo-controlled, study in healthy volunteers. Complement Ther Med 57:102642. https://doi.org/10.1016/j.ctim.2020.102642

Article  PubMed  Google Scholar 

Lopresti AL, Smith SJ (2021) Ashwagandha (Withania somnifera) for the treatment and enhancement of mental and physical conditions: A systematic review of human trials. J Herb Med 28:100434. https://doi.org/10.1016/j.hermed.2021.100434

Article  Google Scholar 

Chandrasekhar K, Kapoor J, Anishetty S (2012) A prospective, randomized double-blind, placebo-controlled study of safety and efficacy of a high-concentration full-spectrum extract of ashwagandha root in reducing stress and anxiety in adults. Indian J Psychol Med 34(3):255–262. https://doi.org/10.4103/0253-7176.106022

CAS  Article  PubMed  PubMed Central  Google Scholar 

Fuladi S, Emami SA, Mohammadpour AH, Karimani A, Manteghi AA, Sahebkar A (2021) Assessment of the efficacy of Withania somnifera root extract in patients with generalized anxiety disorder: a randomized double-blind placebo-controlled trial. Curr Rev Clin Exp Pharmacol 16(2):191–196. https://doi.org/10.2174/1574884715666200413120413

Article  PubMed  Google Scholar 

Deshpande A, Irani N, Balkrishnan R, Benny IR (2020) A randomized, double blind, placebo-controlled study to evaluate the effects of ashwagandha (Withania somnifera) extract on sleep quality in healthy adults. Sleep Med 72:28–36. https://doi.org/10.1016/j.sleep.2020.03.012

Article  PubMed  Google Scholar 

Gopal S, Ajgaonkar A, Kanchi P, Kaundinya A, Thakare V, Chauhan S, Langade D (2021) Effect of an ashwagandha (Withania somnifera) root extract on climacteric symptoms in women during perimenopause: A randomized, double-blind, placebo-controlled study. J Obstet Gynaecol Res 47(12):4414–4425. https://doi.org/10.1111/jog.15030

CAS  Article  PubMed  Google Scholar 

Tharakan A, Shukla H, Benny IR, Tharakan M, George L, Koshy S (2021) Immunomodulatory effect of Withania somnifera (Ashwagandha) extract—a randomized, double-blind, placebo controlled trial with an open label extension on healthy participants. J Clin Med 10(16):3644. https://doi.org/10.3390/jcm10163644

CAS  Article  PubMed  PubMed Central  Google Scholar 

Tiwari S, Gupta SK, Pathak AK (2021) A double-blind, randomized, placebo-controlled trial on the effect of Ashwagandha (Withania somnifera Dunal) root extract in improving cardiorespiratory endurance and recovery in healthy athletic adults. J Ethnopharmacol 272:113929. https://doi.org/10.1016/j.jep.2021.113929

CAS  Article  PubMed  Google Scholar 

Vashi R, Patel BM, Goyal RK (2021) Keeping abreast about Ashwagandha in breast cancer. J Ethnopharmacol 269:113759. https://doi.org/10.1016/j.jep.2020.113759

CAS  Article  PubMed  Google Scholar 

Kulkarni SK, Dhir A (2008) Withania somnifera: an Indian ginseng. Prog Neuropsychopharmacol Biol Psychiatry 32(5):1093–1105. https://doi.org/10.1016/j.pnpbp.2007.09.011

CAS  Article  PubMed  Google Scholar 

Sangwan NS, Tripathi S, Srivastava Y, Mishra B, Pandey N (2017) Phytochemical genomics of Ashwagandha In Kaul SC, Wadhwa R Eds Science of Ashwagandha: preventive and therapeutic potentials. Springer, Cham https://doi.org/10.1007/978-3-319-59192-6_1

Goraya GS, Ved DK (2017) Medicinal Plants in India: An Assessment of their Demand and Supply. National Medicinal Plants Board, Ministry of AYUSH, Government of India. New Delhi and Indian Council of Forestry Research & Education, Dehradun

Google Scholar 

Ved DK, Goraya GS (2008) Demand and supply of medicinal plants. Medplant-ENVIS Newsletter on Medicinal Plants 1(1):2–4

Google Scholar 

Singh VK, Mundkinajeddu D, Agarwal A, Nguyen J, Sudberg S, Gafner S, Blumenthal M (2018) Adulteration of ashwagandha (Withania somnifera) roots and extracts. Botanical Adulterants Prevention Bulletin. ABC-AHP-NCNPR Botanical Adulterants Prevention Program, Austin, TX

Google Scholar 

Mundkinajeddu D, Sawant LP, KoshyR AP, Singh VK, Mayachari A, Sharaf Maged HM, Balasubramanian M, Agarwal A (2014) Development and validation of high performance liquid chromatography method for simultaneous estimation of flavonoid Glycosides in Withania somnifera Aerial Parts. ISRN Anal Chem. https://doi.org/10.1155/2014/351547

Article  Google Scholar 

Chaurasiya ND, Uniyal GC, Lal P, Misra L, Sangwan NS, Tuli R, Sangwan RS (2008) Analysis of withanolides in root and leaf of Withania somnifera by HPLC with photodiode array and evaporative light scattering detection. Phytochem Anal 19(2):148–154. https://doi.org/10.1002/pca.1029

CAS  Article  PubMed  Google Scholar 

Sangwan RS, Chaurasiya ND, Misra LN, Lal P, Uniyal GC, Sharma R, Sangwan NS, Suri KA, Qazi GN, Tuli R (2004) Phytochemical variability in commercial herbal products and preparations of Withania somnifera (Ashwagandha). Curr Sci 86(3):461–465

CAS  Google Scholar 

Amritha N, Bhooma V, Parani M (2020) Authentication of the market samples of Ashwagandha by DNA barcoding reveals that powders are significantly more adulterated than roots. J Ethnopharmacol 256:112725. https://doi.org/10.1016/j.jep.2020.112725

CAS  Article  PubMed  Google Scholar 

Shalini R, Eapen JK, Deepa MS (2017) Macroscopic evaluation of genuine and market samples of Ashwagandha (Withania somnifera (Linn.) Dunal) in Kerala. J Pharmacogn Phytochem 6(6):2283–2288

CAS  Google Scholar 

Upton R, Petrone C, Swisher D (2000) American Herbal Pharmacopoeia: Ashwagandha Root, Withania somnifera: Analytical, Quality Control, and Therapeutic Monograph. Scotts Valley, CA

Google Scholar 

British Pharmacopoeia Commission (2012) Withania somnifera for use in THMP In: The Stationery Office on behalf of the Medicines and Healthcare products Regulatory Agency (Ed), British Pharmacopoeia, London

Government of India Ministry of Health and Family Welfare (2010) Indian Pharmacopoeia. The Indian Pharmacopoeia Commission, Ghaziabad

Google Scholar 

United States Pharmacopeial Convention (2019) Ashwagandha Root; Powdered Ashwagandha Root; and Powdered Ashwagandha Root Extract. In: Pharmacopeia US, Formulary N (eds) Dietary Supplements Compendium. Rockville, MD

Google Scholar 

American Herbal Products Association. Withania somnifera (root). AHPA Botanical Identity References Compendium. http://www.botanicalauthentication.org/index.php/Withania_somnifera_(root). Accessed December 01, 2021.

HPTLC Association Adopted in USP41-N36, Dietary Supplements Compendium 2019.https://www.hptlcassociation.org/media/A48HY22L/Withania_somnifera_Root_HPTLC_Association_USP41-NF36_V2.pdf. Accessed December 01, 2021.

Girme A, Saste G, Pawar S, Balasubramaniam AK, Musande K, Darji B, Satti NK, Verma MK, Anand R, Singh R, Vishwakarma RA (2020) Investigating 11 withanosides and withanolides by UHPLC–PDA and mass fragmentation studies from Ashwagandha (Withania somnifera). ACS Omega 5(43):27933–27943. https://doi.org/10.1021/acsomega.0c03266

CAS  Article  PubMed  PubMed Central  Google Scholar 

Tripathi C, Girme A, Champaneri S, Patel R, Hingorani L (2020) Nutraceutical Regulations: An Opportunity in ASEAN Countries. Nutrition 110729

Kaul MK, Kumar A, Ahuja A, Mir BA, Suri KA, Qazi GN (2009) Production dynamics of withaferin A in Withania somnifera (L.) Dunal complex. Nat Prod Res 23(14):1304–1311. https://doi.org/10.1080/14786410802547440

CAS  Article  PubMed  Google Scholar 

Gupta P, Goel R, Agarwal AV, Asif MH, Sangwan NS, Sangwan RS, Trivedi PK (2015) Comparative transcriptome analysis of different chemotypes elucidates withanolide biosynthesis pathway from medicinal plant Withania somnifera. Sci Rep 5(1):1–3. https://doi.org/10.1038/srep18611

CAS  Article  Google Scholar 

ICH Harmonized Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2 (R1) (2005) International Conference on Harmonization, Geneva

Girme A, Saste G, Pawar S, Ghule C, Mirgal A, Patel S, Tiwari A, Ghoshal S, Bharate SB, Bharate SS, Reddy DS (2021) Quantitative Determination and Characterization of a Kashmir Saffron (Crocus sativus L.)-Based Botanical supplement using single-laboratory validation study by HPLC–PDA with LC–MS/MS and HPTLC investigations. ACS Omega 6(36):23460–23474. https://doi.org/10.1021/acsomega.1c03470

CAS  Article  PubMed  PubMed Central  Google Scholar 

Baby S, John A, Balaji G, Lukose S, Gopakumar B, Koshy KC (2013) UV induced visual cues in grasses. Sci Rep 3(1):2738. https://doi.org/10.1038/srep02738

Article  PubMed  PubMed Central  Google Scholar 

Gauthier-Jaques A, Bortlik K, Hau J, Fay LB (2001) Improved method to track chlorophyll degradation. J Agric Food Chem 49(3):1117–1122. https://doi.org/10.1021/jf000384c

CAS  Article  PubMed  Google Scholar