DMF in SLN can be as effective as free DMF in amelioration of EAE.
•DMF in SLN can reach the same effect of free oral drug with reduced administration.
•DMF in SLNs didn't alter the proportion lymphocyte subsets.
AbstractBackgroundOrally administered dimethyl fumarate (DMF) presents gastrointestinal adverse effects, such as pain and diarrhea, in addition to flushing and lymphopenia.
ObjectiveSolid lipid nanoparticles (SLNs) with DMF were developed for subcutaneous administration.
MethodsDMF-incorporated SLNs and free DMF were tested in mice induced with experimental autoimmune encephalomyelitis (EAE).
ResultsPreventive treatment of free or incorporated DMF were able to reduce the EAE clinical scores, increase the weight of the animals, reduce the lesion area (demyelination and infiltration), reduce microglial fluorescence intensity and reduce the number of microglial cells and astrocytes, when compared to untreated EAE animals. Groups that received DMF had reduced numbers of T cells, B cells and natural killer (NK) cells in the blood, when compared to the non-induced group.
ConclusionsDMF incorporated in SLNs was as effective as free DMF in reducing the clinical scores of the animals, but with reduced administrations when given subcutaneously. In addition, SLN-DMF preventive treatment partially prevented a reduction in the percentages of T and B cells, in the blood, when compared to preventive treatment with free DMF (oral), which suggests reduction of lymphopenia.
KeywordsDimethyl fumarate
Experimental autoimmune encephalomyelitis
Solid lipid nanoparticles
Subcutaneous route
Multiple sclerosis
AbbreviationsSLNssolid lipid nanoparticles
EAEexperimental autoimmune encephalomyelitis
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