Available online 23 August 2022

This study aimed to evaluate the neuroprotective ability of the conditioned medium of stem cells from human exfoliated deciduous teeth (CM-SHED) to prevent glutamate-induced apoptosis of neural progenitors

Neural progenitors were isolated from two-day-old rat brains, and the conditioned medium was obtained from a mesenchymal stem cell SHED. Four groups were examined: neural progenitor cells cultured in neurobasal medium with (N+) and without (N-) glutamate and glycine, and neural progenitor cells cultured in CM-SHED with (K+) and without (K-) glutamate and glycine

The expression of GABA A1 receptor (GABAAR1) messenger RNA (mRNA) in neural progenitor measured by real-time quantitative PCR. GABA contents were measured by enzyme-linked immunosorbent assay, whereas the apoptosis markers caspase-3 and 7-aminoactinomycin D were analysed with a Muse® cell analyzer. The viability of neural progenitor cells in the K+ group (78.05%) was higher than the control group N- (73.22%) and lower in the N+ group (68.90%) than in the control group. The K+ group showed the highest GABA content, which significantly differed from that in the other groups, whereas the lowest content was observed in the N+ group. The expression level of GABAAR1 mRNA in the K+ group was the highest compared to that in the other groups. CM-SHED potently protected the neural progenitors from apoptosis

CM-SHED may effectively prevent glutamate-induced apoptosis of neural progenitors.

secretome

stem cells from human exfoliated deciduous teeth

apoptosis

neural progenitor

gamma-aminobutyric acid

neuroregeneration

analysis of variance

brain derived neurotrophic factor

conditioned medium of stem cells from human exfoliated deciduous teeth

cyclic adenosine monophosphate-response element binding protein

enzyme-linked immunosorbent assay

extracellular signal-regulated kinases

gamma-aminobutyric acid

glutamic acid decarboxylase

glyceraldehyde-3-phospate dehydrogenase

human adipose-derived stem cells

inducible cAMP early repressor

Janus kinase/ signal transducer and activator of transcription

mitogen activated protein kinase

N-methyl-D-aspartate receptor

phosphorylated cyclic adenosine monophosphate-response element binding protein

phosphoinositide-3-kinase

polysialic acid neural cell adhesion molecule

stem cells from human exfoliated deciduous teeth

transforming growth factor

tropomyosin receptor kinase B

© 2022 Published by Elsevier B.V. on behalf of King Saud University.