Induction temperature impacts the structure of recombinant HuGM-CSF inclusion bodies in thermoinducible E. coli

Electronic Journal of Biotechnology

Available online 13 August 2022

Electronic Journal of BiotechnologyAbstractBackground

The temperature upshift has been widely used as an induction system to produce recombinant proteins (RPs). However, thermoinduction could affect bacterial metabolism, RP production, and RP aggregation. Understanding the structure and functionality of those aggregates, known as inclusion bodies (IBs), is a research area of interest in bioprocesses being scarcely studied under thermoinduction. Here, we describe the effect of the thermoinduction (39°C or 42°C) on the production of the recombinant human granulocyte-macrophage colony-stimulating factor (rHuGM-CSF) using Escherichia coli W3110 under the system λpL/cI857.

Results

Results indicated that at 39°C, the production of biomass was almost doubled as well as the acetate accumulation compared to 42°C. Cultures thermoinduced at 42°C improved 1.5-fold the total protein over biomass yield and 1.25-fold the RP over total protein yield. Furthermore, 42°C accelerated the onset of IBs formation, changing its architecture. Additionally, IBs formed at 42°C were less soluble and presented higher disorderly structures compared with IBs formed at 39°C, enriched in α-helix and amyloidal-like structures.

Conclusions

This study highlights the observation that IBs attain different architecture in response to small changes in environmental conditions, such as the induction temperature, being this helpful information to improve thermoinduced bioprocesses.

Keywords

Acetate

Aggregation

Biomass

Bioprocesses

Escherichia coli

Inclusion bodies

Induction

Recombinant human granulocyte-macrophage colony-stimulating factor

Recombinant proteins

Temperature upshift

Thermoinduction

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