Masaya Hattori,1 Atsuko Yabuuchi,1 Hayate Tanaka,1 Taketo Kawara,1 Hongyan Wang,1 Koji Inoue,2 Shunichi Shiozawa,3 Koichiro Komai1

1 Division of Medical Biophysics, Department of Biophysics, Graduate School of Health Sciences, Kobe University, Kobe, Japan
2 Shichikawa Arthritis Research Center, Osaka Rehabilitation Hospital, Hannan, Japan
3 Institute for Rheumatic Diseases, Ashiya, Japan

Abstract

Background: Palindromic rheumatism (PR) is an infrequent form of periodic arthritis. Based on the similarity of the pathogenesis of PR to autoinflammatory syndromes, we previously found that the dominant-active splice variant of the inflammasome adaptor protein, apoptosis-associated speck-like protein containing a CARD (ASC), which lacks exon 2 (Δexon2), is expressed in Japanese patients with PR.
Objective: Elucidation of the mechanism of Δexon2 ASC production and the effect of IL-1β on splicing.
Methods: The genomic DNA of Japanese patients with PR was sequenced. The effect of the observed single nucleotide polymorphisms (SNPs) on ASC splicing was determined via exon trapping using THP-1 cells stimulated with interleukin-1 beta (IL-1β) or ceramide. To investigate the genes that affect alternative splicing via IL-1β, we analyzed the transcriptome of IL-1β-treated THP-1 cells using RNA sequencing.
Results: We found the rs8056505 A→G SNP located in the 5’-untranslated region of the genomic ASC gene in patients and that Δexon2 expression was induced by this SNP, whereas it was suppressed by IL-1β or ceramide. We detected 131,426 transcripts and identified 52 differentially expressed genes (DEGs) consisting of 41 downregulated genes and 11 upregulated genes in IL-1β-stimulated THP-1 cells. The splicing-related gene MASCRNA was the most significantly induced gene by IL-1β.
Conclusion: We propose a cyclic expression model in which ASC alternates between wild-type and Δexon2 expression regulated by the rs8056505 G allele and splicing factors induced by IL-1β. This cycle may be correlated with the formation of periodic PR pathologies.
Key words: palindromic rheumatism, inflammasome, ASC, splice variant, IL-1β