Ultrarapid Targeted Nanopore Sequencing for Fusion Detection of Leukemias

Abstract

Acute leukemia (AL), a cancer of the blood, has continued high mortality rates despite a plethora of available treatments. Variable prognosis, treatment response and survival are largely based on distinct cytogenetic and molecular aberrations that characterize AL subtypes. Fast and cost-effective methods of detecting AL fusions and mutations are needed to improve clinical outcomes for patients. We report preliminary data on an ultrarapid and portable nanopore long-read sequencing assay with a same-day turnaround time of ~6-7 hours for identifying key leukemic fusions. Leukemias frequently present with recurrent fusions that impact risk stratification and therapy choice, our assay can help oncologists overcome time related challenges to get patients on treatments faster.

Competing Interest Statement

The authors have declared no competing interest.

Funding Statement

Funding is provided in part by NCCN young investigator award and the Hyundai Hope on Wheels Scholars Award, R01 CA175008-06, UG1 CA233338-02, R01GM126019, 75N91021C00022, and 75N91020C00009.

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The authors collectively declare that this research is conducted with approval from the Fred Hutch institutional review board and meets the requirements for ethics approval and consent to participate and consent for publication.

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Yes

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Data Availability

Data is available for review upon request to the corresponding author and the data will be made publicly available upon acceptance.

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