Mesorhizobium ventifaucium sp. nov. and Mesorhizobium escarrei sp. nov., two novel root-nodulating species isolated from Anthyllis vulneraria

Elsevier

Available online 16 June 2022, 126341

Systematic and Applied MicrobiologyAbstract

Ten mesorhizobial strains isolated from root-nodules of Anthyllis vulneraria by trapping using soils from southern France were studied to resolve their taxonomy. Their 16S rDNA sequences were identical and indicated that they are affiliated to the genus Mesorhizobium within the group M. prunaredense/M. delmotii/M. temperatum/M. mediterraneum/M. wenxiniae and M. robiniae as the closest defined species. Their evolutionary relationships with validated species were further characterized by multilocus sequence analysis (MLSA) using 4 protein-coding housekeeping genes (recA, atpD, glnII and dnaK), that divides the strains in two groups, and suggest that they belong to two distinct species. These results were well-supported by MALDI-TOF mass spectrometry analyses, wet-lab DNA-DNA hybridization (≤58%), and genome-based species delineation methods (ANI<96%, in silico DDH<70%), confirming their affiliation to two novel species. Based on these differences, Mesorhizobium ventifaucium (STM4922T=LMG 29643T =CFBP 8438T) and Mesorhizobium escarrei (type strain STM5069T =LMG 19642T =CFBP 8439T) are proposed as names for these two novel species. The phylogeny of nodulation genes nodC and nodA allocated the type strains into symbiovar anthyllidis as well as those of M. metallidurans STM2683T, M. delmotii STM4623T and M. prunaredense STM4891T, all recovered from the same legume species.

AbbreviationsMLSA

multilocus sequence analysis

ANI

average nucleotide identity

CEC

cation exchange capacity

YEM

yeast extract mannitol

MALDI-TOF MS

matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

HPLC

high performance liquid chromatography

CTAB

cetyl trimethyl ammonium bromide

ENA

European nucleotide archive

GGDC

genome-to-genome distance calculator

TYGS

type (strain) genome server

GDDP

genome blast distance phylogeny approach

BLAST

basic local alignment search tool

LMG

Laboratory of Microbiology of Ghent

CFBP

Collection Française de Bactéries associées aux Plantes)

STM

(Symbioses Tropicales et Méditerranéennes)

Keywords

Mesorhizobium sp. nov.

Rhizobia

Symbiosis

Taxonomy

Phylogeny

Symbiovar

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