Available online 16 June 2022, 126341
AbstractTen mesorhizobial strains isolated from root-nodules of Anthyllis vulneraria by trapping using soils from southern France were studied to resolve their taxonomy. Their 16S rDNA sequences were identical and indicated that they are affiliated to the genus Mesorhizobium within the group M. prunaredense/M. delmotii/M. temperatum/M. mediterraneum/M. wenxiniae and M. robiniae as the closest defined species. Their evolutionary relationships with validated species were further characterized by multilocus sequence analysis (MLSA) using 4 protein-coding housekeeping genes (recA, atpD, glnII and dnaK), that divides the strains in two groups, and suggest that they belong to two distinct species. These results were well-supported by MALDI-TOF mass spectrometry analyses, wet-lab DNA-DNA hybridization (≤58%), and genome-based species delineation methods (ANI<96%, in silico DDH<70%), confirming their affiliation to two novel species. Based on these differences, Mesorhizobium ventifaucium (STM4922T=LMG 29643T =CFBP 8438T) and Mesorhizobium escarrei (type strain STM5069T =LMG 19642T =CFBP 8439T) are proposed as names for these two novel species. The phylogeny of nodulation genes nodC and nodA allocated the type strains into symbiovar anthyllidis as well as those of M. metallidurans STM2683T, M. delmotii STM4623T and M. prunaredense STM4891T, all recovered from the same legume species.
AbbreviationsMLSAmultilocus sequence analysis
ANIaverage nucleotide identity
CECcation exchange capacity
YEMyeast extract mannitol
MALDI-TOF MSmatrix-assisted laser desorption/ionization time-of-flight mass spectrometry
HPLChigh performance liquid chromatography
CTABcetyl trimethyl ammonium bromide
ENAEuropean nucleotide archive
GGDCgenome-to-genome distance calculator
TYGStype (strain) genome server
GDDPgenome blast distance phylogeny approach
BLASTbasic local alignment search tool
LMGLaboratory of Microbiology of Ghent
CFBPCollection Française de Bactéries associées aux Plantes)
STM(Symbioses Tropicales et Méditerranéennes)
KeywordsMesorhizobium sp. nov.
Rhizobia
Symbiosis
Taxonomy
Phylogeny
Symbiovar
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