Exosomal proteomics in kidney disease: From technical approaches to clinical applications

 

Authors

Ageng Brahmadhi, Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
Yung-Kun Chuang, School of Food Safety, Taipei Medical University, Taipei, Taiwan
San-Yuan Wang, Master Program for Clinical Pharmacogenomics and Pharmacoproteomics, College of Pharmacy, Taipei Medical University, Taipei, Taiwan
Chih-Chin Kao, Division of Nephrology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan
I-Lin Tsai, Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, 250 Wuxing Street, Taipei 11031, TaiwanFollow

Abstract

Exosomes are small extracellular vesicles (sEVs) secreted from cells and have a general diameter ranging from 30~150 nm. It was reported that exosomes have essential roles in intercellular communication and can be targeted as biomarkers of disease or as therapeutic agents. Among the different techniques used for exosome investigation, the mass spectrometry-based proteomics approach has accelerated the unraveling of the molecular composition of exosomes and has contributed to improved knowledge of molecular processes in various diseases. In this review, we focused on proteomics-based studies of exosomes and clinical applications in kidney diseases. A general introduction of exosomes, isolation and characterization techniques, and proteomics-based study workflows are included in this article. We also categorized applications in acute kidney injury, chronic kidney disease, renal transplantation, congenital kidney disease, and malignant kidney disorder to show the important findings from proteomics-based exosomal investigations.

Recommended Citation

Brahmadhi, Ageng; Chuang, Yung-Kun; Wang, San-Yuan; Kao, Chih-Chin; and Tsai, I-Lin (2022) "Exosomal proteomics in kidney disease: From technical approaches to clinical applications," Journal of Food and Drug Analysis: Vol. 30 : Iss. 2 , Article 3.
Available at: https://doi.org/10.38212/2224-6614.3409

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