aDepartment of Molecular Pathology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
bDepartment of Pathology, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam
cDepartment of Urology, Hiroshima University Graduate School of Biomedical and Health Sciences, Hiroshima, Japan
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Article / Publication DetailsFirst-Page Preview
Received: November 16, 2021
Accepted: February 06, 2022
Published online: June 09, 2022
Number of Print Pages: 12
Number of Figures: 4
Number of Tables: 3
ISSN: 1015-2008 (Print)
eISSN: 1423-0291 (Online)
For additional information: https://www.karger.com/PAT
AbstractIntroduction: Tryptophan metabolism has been shown to be involved in tumor development. Two main tryptophan-degrading enzymes, tryptophan 2,3-dioxygenase (TDO2) and indoleamine 2,3-dioxygenase 1 (IDO1), may potently promote cancer cell survival and distant metastasis in diverse types of cancer, such as lung and breast cancer. IDO1 overexpression is an independent prognosticator in gastric cancer (GC). This work aimed to uncover the expression of TDO2 and its clinicopathologic significance in GC. Methods: TDO2 expression was evaluated in public data of The Cancer Genome Atlas cohort STAD and in two different GC cohorts. Correlation between TDO2 and immune cell infiltrates as well as PD-L1 tumor staining was investigated. The biofunction of TDO2 was examined with MTT, colony formation, and spheroid formation assays by RNA interference. Results: TDO2 expression was correlated with both progressive disease and clinical outcome, and its expression was an independent predictor of prognosis in GC. TDO2 expression was correlated with infiltration of immune cells and tumor expression of PD-L1. Inhibition of TDO2 expression suppressed cell proliferation, colony formation, and cell invasion of GC cells. Additionally, suppression of TDO2 expression inhibited spheroid body-formation and viability of GC organoids. Conclusion: Our data show that TDO2 might be a crucial marker for predicting prognosis and targeted therapy in GC.
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Received: November 16, 2021
Accepted: February 06, 2022
Published online: June 09, 2022
Number of Print Pages: 12
Number of Figures: 4
Number of Tables: 3
ISSN: 1015-2008 (Print)
eISSN: 1423-0291 (Online)
For additional information: https://www.karger.com/PAT
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