Endophytic fungi are associated within plant tissues without any harmful effects. They produce a diversity of metabolites and are well known as rich source of bioactive substances. This study aims to identify fungal isolates from different medicinal plants, evaluate their antioxidant potency and detect components responsible for antioxidant activity.

In the present investigation, 4 endophytic fungal isolates were obtained from different medicinal plants Rumex nervosus, Pulicaria crispa and Withania somnifera. Those isolates were characterized as Penicilluim commune, P. glaucoroseum, Aspergillus flavipes and Fusarium chlamydosporum according to morphological and phylogenetic basis. These strains were propagated on humified rice and their crude ethyl acetate extracts were assayed for their total phenols, flavonoids and antioxidant potency against DPPH free radicals. The results showed that, the elevated constituent of phenolics was found in P. commune (148.24 mg/g), followed by F. chlamydosporum (124.25 mg/g), while the highest total flavonoids were determined in A. flavipes, followed by F. chlamydosporum, P. glaucoroseum, P. commune, respectively. Moreover, the maximum hydroxyl radical-scavenging assay was IC50 = 2.13 mg/mL exhibited by P. commune, followed by F. chlamydosporum (2.68 mg/mL). Weak antimicrobial and cytotoxic effects were reported by the studied strains against pathogenic isolates and (HepG2) hepatocellular carcinoma cell line. The fungal EtOAc extracts were characterized quantitatively by the incidence of different phenols, flavonoids, and phenolic acids, as detected by HPLC.