A composite of hepatocyte growth factor and 5α-dihydrotestosterone–gelatin microspheres with adipose-derived stem cells enhances wound healing

Skin Pharmacology and Physiology

Tao K. · Bai X. · Ji P. · Zhang Y. · Cao T. · Han F. · Zhang Z. · Guan H. · Hu D.

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Article / Publication Details Abstract

Introduction: Reconstructing sebaceous glands is one goal of functionally healing patients who have suffered severe burns, instead of the simple pursuit of wound closure. Effective regeneration of skin appendages remains a challenge in skin wound management and research. Objective: The aim of this study was to evaluate the differentiation of ADSC into sebaceous glands, and clarified the involvement of HGF and 5α-DHT in this process. Methods: This study used hepatocyte growth factor (HGF)– and 5α-dihydrotestosterone (5α-DHT)–gelatin microspheres to treat human adipose-derived stem cells (ADSCs) and investigated the reconstruction of sebaceous glands. HGF– and 5α-DHT–gelatin microspheres were constructed using microcapsule slow-release technology. Mice full-thickness skin-wound model was established to evaluate wound healing and hematoxylin-eosin staining was utilized to determine the skin structure. Results: In vitro analyses found that HGF– and 5α-DHT–gelatin microspheres promoted migration of and tube formation by ADSCs. Furthermore, AKT/ERK signaling, which is related to sebocyte and sweat gland epithelial cell growth, were activated after HGF and 5α-DHT treatment. An in vivo wound healing model demonstrated that ADSCs primed with amnion-loaded HGF– and 5α-DHT–gelatin microspheres promoted wound healing and increased sebaceous gland formation compared to the control group. Conclusions: This study confirms the efficacy of ADSCs treated with amnion and HGF– and 5α-DHT–gelatin microspheres in accelerating wound healing and effectively restoring sebaceous glands. This engineered tissue provides insight into and a novel therapeutic material for burns and full-thickness skin wounds.¬¬

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