Cytogenetic and Genome Research

Farias J.C.a· Santos N.a· Bezerra D.P.a· Sotero-Caio C.G.a,b

Author affiliations

aDepartamento de Genética, Centro de Biociências, UFPE- Universidade Federal de Pernambuco, Recife, Brazil
bWellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridgeshire, United Kingdom

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Article / Publication Details

First-Page Preview

Received: September 01, 2021
Accepted: November 16, 2021
Published online: January 28, 2022

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

The subfamily Lonchorhininae encompasses 6 species of sword-nosed bats (Lonchorhina) and is one of the most problematic lineages in the Neotropical leaf-nosed bats (Phyllostomidae) phylogeny. There are at least 5 different hypotheses to explain when the subfamily diverged from the remaining phyllostomids, but none with robust statistical support. Here, we generated a chromosome painting homology map of Lonchorhina aurita karyotype (2n = 32 and FN = 60) using whole-chromosome probes of Macrotus californicus (MCA; 2n = 40 and FN = 60). We placed the karyotype changes of L. aurita in a phylogenetic context to discuss the most likely branching position of Lonchorhininae based on karyotypic evolution. We show that L. aurita has a derived karyotype with 24 segments homologous to the 20 MCA chromosomes used as probes. Comparative analyses between 7 published painted bats species across 4 phyllostomid subfamilies (Macrotinae, Phyllostominae, Glossophaginae, and Lonchophyllinae) revealed that one inversion (MCA 4inv) and one fusion (MCA 17 + 18) are shared derived features between the karyotypes of L. aurita and species of Phyllostominae not yet observed in other bats. Our data show that chromosomal homology maps may contribute with new insights into a long-standing phylogenetic debate that has endured for decades.

© 2022 S. Karger AG, Basel

References Amador LI, Arévalo RLM, Almeida FC, Catalano SA, Giannini NP. Bat systematics in the light of unconstrained analyses of a comprehensive molecular supermatrix. J Mammal Evol. 2018;25:37–70. Baker HDR. The Family in State and Society. In: Baker RJ, Jones JK, Carter DC, editors. Biology of bats of the New World family Phyllostomatidae Part III. Special Publications Mus Texas Tech Univ; 1979. 16. p. 107–35. Baker RJ, Bickham JW. Karyotypic evolution in bats: Evidence of extensive and conservative chromosomal evolution in closely related taxa. Systematic Biology. 1980;29(3):239–53. Baker RJ, Hoofer SR, Porter CA, Van Den Bussche RA. Diversification among New World leaf-nosed bats: an evolutionary hypothesis and classification inferred from digenomic congruence of DNA sequence. Occas Papers Mus Texas Tech Univ. 2003a;230:1–32. Baker RJ, Hamilton MJ, Parish DA. Preparations of mammalian karyotypes under field conditions. Occas Papers Mus Texas Tech Univ. 2003b;228:1–8. Baker RJ, Solari S, Cirranello A, Simmons NB. Higher level classification of phyllostomid bats with a summary of DNA synapomorphies. Acta Chiropterologica. 2016;18(1):1–38. Barros HMDR, Sotero-Caio CG, Santos N, Souza MJ. Comparative cytogenetic analysis between Lonchorhina aurita and Trachops cirrhosus (Chiroptera, Phyllostomidae). Genet Mol Biol. 2009;32:748–52. Benathar TCM, Nagamachi CY, Rodrigues LRR, O’Brien PCM, Ferguson-Smith MA, Yang F, et al. Karyotype, evolution and phylogenetic reconstruction in Micronycterinae bats with implications for the ancestral karyotype of Phyllostomidae. BMC Evol Biol. 2019;19:98. Botero-Castro F, Tilak M, Justy F, Catzeflis F, Delsuc F, Douzery EJP. In Cold Blood: Compositional Bias and Positive Selection Drive the High Evolutionary Rate of Vampire Bats Mitochondrial Genomes. Genome Biol Evol. 2018;10:2218–39. Carbone L, Harris RA, Gnerre S, Veeramah KR, Lorente-Galdos B, Huddleston J, et al. Gibbon genome and the fast karyotype evolution of small apes. Nature. 2014;513:195–201. Dávalos LM, Velazco PM, Warsi OM, Smits PD, Simmons NB. Integrating incomplete fossils by isolating conflicting signal in saturated and non-independent morphological characters. Syst Biol. 2014;63:582–600. Deakin JE, Potter S, O’Neill R, Ruiz-Herrera A, Cioffi MB, Eldridge MDB, et al. Chromosomics: Bridging the Gap between Genomes and Chromosomes. Genes (Basel). 2019;10:627. Dumont ER, Dávalos LM, Goldberg A, Santana SE, Rex K, Voigt CC. Morphological innovation, diversification and invasion of a new adaptive zone. Proc Biol Sci. 2012;279:1797–805. Ferguson-Smith MA, Trifonov V. Mammalian karyotype evolution. Nat Rev Genet. 2007;8:950–62. Gessinger G, Gonzalez-Terrazas TP, Page RA, Jung K, Tschapka M. Unusual echolocation behaviour of the common sword-nosed bat Lonchorhina aurita: an adaptation to aerial insectivory in a phyllostomid bat? R Soc Open Sci. 2019;6:182165. Gessinger G, Page R, Wilfert L, Surlykke A, Brinkløv S, Tschapka M. Phylogenetic Patterns in Mouth Posture and Echolocation Emission Behavior of Phyllostomid Bats. Front Ecol Evol. 2021;9:630481. Graphodatsky A, Ferguson-Smith MA, Stanyon R. A short introduction to cytogenetic studies in mammals with reference to the present volume. Cytogenet Genome Res. 2012;137:83–96. Hedges DJ, Deininger PL. Inviting instability: Transposable elements, double-strand breaks, and the maintenance of genome integrity. Mutat Res. 2007;616:46–59. Mantilla-Meluk H, Perez-Amaya N, Velazco PM. Morphometric variation of the genus Lonchorhina (Chiroptera, Lonchorhininae), with noteworthy comments on taxonomy and distributional range extensions. In: Bradley RD, Genoways HH, Schmidly DJ, Bradley LC, editors. From field to laboratory: a memorial volume in honor of Robert J. Baker. Special Publications Mus Texas Tech Univ; 2019. 71. p. 149–68. Pieczarka JC, Nagamachi CY, O’Brien PC, Yang F, Rens W, Barros RMS, et al. Reciprocal chromosome painting between two South American bats: Carollia brevicauda and Phyllostomus hastatus (Phyllostomidae, Chiroptera). Chromosom Res. 2005;13:339–47. Ribas TFA, Rodrigues LRR, Nagamachi CY, Gomes AJB, Rissino JD, O’Brien PCM, et al. Phylogenetic reconstruction by cross-species chromosome painting and G-banding in four species of Phyllostomini tribe (Chiroptera, Phyllostomidae) in the Brazilian Amazon: An independent evidence for monophyly. PLoS One. 2015;10:e0122845. Rojas D, Vale A, Ferrero V, Navarro L. When did plants become important to leaf-nosed bats? Diversification of feeding habits in the family Phyllostomidae. Mol Ecol. 2011;20:2217–28. Rojas D, Warsi OM, Dávalos LM. Bats (Chiroptera: Noctilionoidea) challenge a recent origin of extant Neotropical diversity. Syst Biol. 2016;65:432–48. Rokas A, Holland PW. Rare genomic changes as a tool for phylogenetics. Trends Ecol Evol. 2000;15:454–9. Seabright M. A rapid banding technique for human chromosomes. Lancet. 1971;2:971–2. Simmons NB, Cirranello AL. Bat Species of the World: A Taxonomic and Geographic Database; 2020. Available from: https://batnames.org/. Solari S, Sotero-Caio CG, Baker RJ. Advances in systematics of bats: towards a consensus on species delimitation and classifications through integrative taxonomy. J Mammal. 2019;100(3):838–51. Sotero-Caio CG, Volleth M, Gollahon LS, Fu B, Cheng W, Ng BL, et al. Chromosomal evolution among leaf-nosed nectarivorous bats-evidence from cross-species chromosome painting (Phyllostomidae, Chiroptera). BMC Evol Biol. 2013;13:276. Sotero-Caio CG, Volleth M, Hoffmann FG, Scott L, Wichman HA, Yang F, et al. Integration of molecular cytogenetics, dated molecular phylogeny, and model-based predictions to understand the extreme chromosome reorganization in the Neotropical genus Tonatia (Chiroptera: Phyllostomidae). BMC Evol Biol. 2015;15:220. Sotero-Caio CG, Baker RJ, Volleth M. Chromosomal Evolution in Chiroptera. Genes (Basel). 2017;8:272. Volleth M, Heller KG, Pfeiffer RA, Hameister H. A comparative ZOO-FISH analysis in bats elucidates the phylogenetic relationships between Megachiroptera and five microchiropteran families. Chromosome Res. 2002;10:477–97. Volleth M, Son NT, Wu Y, Li Y, Yu W, Lin LK, et al. Comparative chromosomal studies in Rhinolophus formosae and R. luctus from China and Vietnam: Elevation of R. l. lanosus to species rank. Acta Chiropt. 2017;19:41–50. Wetterer AL, Rockman MV, Simmons NB. Phylogeny of phyllostomid bats (Mammalia: Chiroptera): data from diverse morphological systems, sex chromosomes, and restriction sites. B Am Mus Nat Hist. 2000;248:1–200. Article / Publication Details

First-Page Preview

Received: September 01, 2021
Accepted: November 16, 2021
Published online: January 28, 2022

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 1

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

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