Novel Centromeric and Subtelomeric Repetitive DNA Sequences for Karyotyping the Bambara Groundnut (Vigna subterranea L. Verdc.)

Cytogenetic and Genome Research

Tek A.L. · Kara Öztürk S.D. · Yıldız H. · Karalar D.

Author affiliations

Department of Agricultural Genetic Engineering, Ayhan Şahenk Faculty of Agricultural Sciences and Technologies, Niğde Ömer Halisdemir University, Niğde, Turkey

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Article / Publication Details

First-Page Preview

Abstract of Original Article

Received: September 28, 2021
Accepted: December 07, 2021
Published online: January 12, 2022

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

Bambara groundnut (Vigna subterranea L. Verdc.) is an un­derutilized minor legume crop with climate resilience and great potential use in world agriculture. This study aimed to cytogenetically characterize the genome and chromosome properties of Bambara groundnut. We cloned, sequenced, and mapped a 50-bp centromere-specific tandem repeat on all chromosomes. In addition, a 400-bp subtelomeric repeat was discovered and mapped on a single pair of chromosomes. A Bambara groundnut karyotype was constructed using these novel repeats along with ribosomal RNA genes (45S and 5S) and telomeric DNA sequences. This study provides the first analysis of the genome and chromosome properties of Bambara groundnut. We discuss our findings in relation to genetic improvement of Bambara groundnut and centromere evolution in legume species.

© 2022 S. Karger AG, Basel

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First-Page Preview

Abstract of Original Article

Received: September 28, 2021
Accepted: December 07, 2021
Published online: January 12, 2022

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 0

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

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