Cytogenetic and Genome Research

Novaes C.M.a· Cunha M.S.a· Werneck H.A.a· Fernandes A.a,b· Campos L.A.O.a· Lopes D.M.a

Author affiliations

aLaboratório de Citogenética de Insetos, Departamento de Biologia Geral, Universidade Federal de Viçosa, Viçosa, Brazil
bDepartamento de Ciências Biológicas, Universidade do Estado de Mato Grosso, Tangará da Serra, Brazil

Log in to MyKarger to check if you already have access to this content.

Buy FullText & PDF Unlimited re-access via MyKarger Unrestricted printing, no saving restrictions for personal use read more

CHF 38.00 *
EUR 35.00 *
USD 39.00 *

Select

KAB

Buy a Karger Article Bundle (KAB) and profit from a discount!

If you would like to redeem your KAB credit, please log in.

Save over 20% compared to the individual article price.

Learn more

Rent/Cloud Rent for 48h to view Buy Cloud Access for unlimited viewing via different devices Synchronizing in the ReadCube Cloud Printing and saving restrictions apply Rental: USD 8.50
Cloud: USD 20.00

Select

Subscribe Access to all articles of the subscribed year(s) guaranteed for 5 years Unlimited re-access via Subscriber Login or MyKarger Unrestricted printing, no saving restrictions for personal use read more

Subcription rates

Select

* The final prices may differ from the prices shown due to specifics of VAT rules.

Article / Publication Details

First-Page Preview

Received: September 22, 2021
Accepted: October 28, 2021
Published online: December 17, 2021

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 2

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Abstract

The genus Partamona includes 33 species of stingless bees, of which 11 were studied cytogenetically. The main goal of this study was to propose a hypothesis about chromosomal evolution in Partamona by combining molecular and cytogenetic data. Cytogenetic analyses were performed on 3 Partamona species. In addition, the molecular phylogeny included mitochondrial sequences of 11 species. Although the diploid number was constant within the genus, 2n = 34, B chromosomes were reported in 7 species. Cytogenetic data showed karyotypic variations related to chromosome morphology and the amount and distribution of heterochromatin and repetitive DNA. The molecular phylogenetic reconstruction corroborated the monophyly of the genus and separated the 2 clades (A and B). This separation was also observed in the cytogenetic data, in which species within each clade shared most of the cytogenetic characteristics. Furthermore, our data suggested that the B chromosome in the genus Partamona likely originated from a common ancestor of the species that have it in clade B and, through interspecific hybridization, it appeared only in Partamona rustica from clade A. Based on the above, Partamona is an interesting genus for further investigations using molecular mapping of B chromosomes as well as for broadening phylogenetic data.

© 2021 S. Karger AG, Basel

References Batalha-Filho H, Waldschmidt AM, Campos LAO, Tavares MG, Fernandes-Salomão TM. Phylogeography and historical demography of the neotropical stingless bee Melipona quadrifasciata (Hymenoptera, Apidae): Incongruence between morphology and mitochondrial DNA. Apidologie. 2010;41:534–47. Brito RM, Costa MA, Pompolo SG. Characterization and distribution of supernumerary chromosomes in 23 colonies of Partamona helleri (Hymenoptera, Apidae, Meliponinae). Braz J Genet. 1997;20:185–8. Brito RM, Caixeiro APA, Pompolo SG, Azevedo GG. Cytogenetic data of Partamona peckolti (Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/CMA3 and DA/DAPI. Genet Mol Biol. 2003;26:53–7. Brito RM, Pompolo SG, Magalhães MFM, Barros EG, Hojo ETS. Cytogenetic characterization of two Partamona species (Hymenoptera, Apinae, Meliponini) by fluorochrome staining and localization of 18S rDNA clusters by FISH. Cytologia. 2005;70:373–80. Brito-Ribon RM, Miyazawa CS, Pompolo SG. First karyotype characterization of four species of Partamona (Friese, 1980) (Hymenoptera, Apidae, Meliponini) in Mato Grosso State - Brazil. Cytobios. 1999;100:19–26. Camacho JPM. B chromosomes. In: Gregory TR, editor. The evolution of the genome. Academic Press; 2005. p. 223–86. Camacho JP, Sharbel TF, Beukeboom LW. B-chromosome evolution. Philos Trans R Soc Lond B Biol Sci. 2000;355:163–78. Camargo JMF, Pedro SEM. Meliponini neotropicais: o gênero Partamona Schwarz, 1939 (Hymenoptera, Apidae, Apinae) - bionomia e biogeografia. Rev Bras Entomol. 2003;47:311–72. Cardoso DC, Pompolo SG, Cristiano MP, Tavares MG. The role of fusion in ant chromosome evolution: insights from cytogenetic analysis using a molecular phylogenetic approach in the genus Mycetophylax. PLoS One. 2014;9:e87473. Cardoso DC, Heinze J, Moura MN, Cristiano MP. Chromosomal variation among populations of a fungus-farming ant: implications for karyotype evolution and potential restriction to gene flow. BMC Evol Biol. 2018;18:146. Correia AM, Fernandes A, Campos LAO, Lopes DM. Análise da presença do marcador SCAR associado ao cromossomo B em espécies de abelha sem ferrão do gênero Partamona. Rev Bras Biocienc. 2014;12:196–200. Cunha MS, Cardoso DC, Cristiano MP, Campos LAO, Lopes DM. The Bee Chromosome Database (Hymenoptera: Apidae). Apidologie. 2021a;52:493–502. Cunha MS, Soares FAF, Clarindo WR, Campos LAO, Lopes DM. Robertsonian rearrangements in Neotropical Meliponini karyotype evolution (Hymenoptera: Apidae: Meliponini). Insect Mol Biol. 2021b;30:379–89. Diniz D, Melo PX. Easy Idio, version 1.0. 2006. Fusu L. An integrative taxonomic study of European Eupelmus (Macroneura) (Hymenoptera: Chalcidoidea: Eupelmidae), with a molecular and cytogenetic analysis of Eupelmus (Macroneura) vesicularis: several species hiding under one name for 240 years. Zool J Linn Soc. 2017;181:519–603. Gokhman VE, Kuznetsova VG. Comparative insect karyology: current state and applications. Entmol Rev. 2006;86:352–68. Gonçalves GC, Dalbosco AM, Barth A, Miranda EA, Costa MA. Comparative cytogenetic analysis of three species of the genus Partamona (Apidae, Meliponini). Apidologie. 2020;52:80–8. Hasterok R, Wolny E, Hosiawa M, Kowalczyk M, Kulak-Ksiazczyk S, Ksiazczyk T, et al. Comparative analysis of rDNA distribution in chromosomes of various species of Brassicaceae. Ann Bot. 2006;97:205–16. Hoshiba H, Imai H. Chromosome evolution of bees and wasps (Hymenoptera, Apocrita) on the basis of C-banding pattern analyses. Jpn J Entomol. 1993;61:465–92. Imai HT, Taylor RW, Crosland MW, Crozier RH. Modes of spontaneous chromosomal mutation and karyotype evolution in ants with reference to the minimum interaction hypothesis. Jpn J Genet. 1988;63:159–85. Imai HT, Brown WLJ, Kubota M, Yong HS, Tho YP. Chromosome observations on tropical ants from western Malaysia. Annu Rep Natl Inst Genet. 1994;34:66–9. Kasahara S, Zampieri Silva AP, Gruber SL, Haddad CF. Comparative cytogenetic analysis on four tree frog species (Anura, Hylidae, Hylinae) from Brazil. Cytogenet Genome Res. 2003;103:155–62. Labaroni CA, Malleret MM, Novillo A, Ojeda A, Rodriguez D, Cuello P, et al. Karyotypic variation in the Andean rodent Phyllotis xanthopygus (Waterhouse, 1837) (Rodentia, Cricetidae, Sigmodontinae). Comp Cytogenet. 2014;8:369–81. Levan A, Fredga K, Sandberg AA. Nomenclature for centromeric position on chromosomes. Hereditas. 1964;52:201–20. Lopes DM, Travenzoli NM, Fernandes A, Campos LAO. Different levels of chromatin condensation in Partamona chapadicola and Partamona nhambiquara (Hymenoptera, Apidae). Cytogenet Genome Res. 2020;160:206–13. Machado DP, Miranda EA, Dessi MC, Sabadini CP, Del Lama MA. Occurrence and origin of supernumerary chromosomes in Partamona (Hymenoptera: Apidae: Meliponini). Cytogenet Genome Res. 2016;150:68–75. Marthe JB, Pompolo SG, Campos LAO, Salomão TMF, Tavares MG. Cytogenetic characterization of Partamona cupira (Hymenoptera, Apidae) by fluorochromes. Genet Mol Biol. 2010;33:253–5. Martins CC, Duarte OMP, Waldschmidt AM, Alves RMO, Costa MA. New occurrence of B chromosomes in Partamona helleri (Friese, 1900) (Hymenoptera, Meliponini). Genet Mol Biol. 2009;32:782–5. Martins CCC, Diniz D, Sobrinho-Scudeler PE, Foresti F, Campos LAO, Costa MA. Investigation of Partamona helleri (Apidae, Meliponini) B chromosome origin. An approach by microdissection and whole chromosome painting. Apidologie. 2013;44:75–81. Martins CCC, Waldschmidt AM, Costa MA. Unprecedented record of ten novel B chromosomes in the stingless bee Partamona helleri (Apidae, Meliponini). Apidologie. 2014;45:431–9. Menezes RST, Brady SG, Carvalho AF, Del Lama MA, Costa MA. The roles of barriers, refugia, and chromosomal clines underlying diversification in Atlantic Forest social wasps. Sci Rep. 2017;7:7689. Micolino R, Cristiano MP, Travenzoli NM, Lopes DM, Cardoso DC. Chromosomal dynamics in space and time: evolutionary history of Mycetophylax ants across past climatic changes in the Brazilian Atlantic coast. Sci Rep. 2019;9:18800. Miller MA, Pfeiffer W, Schwartz T. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In. Proceedings of the Gateway Computing Environments Workshop (GCE). New Orleans, LA; 2010. Paladino LC, Muntaabski I, Lanzavecchia S, Bagousse-Pinguet YL, Viscarret M, Juri M, et al. Complementary sex determination in the parasitic wasp Diachasmimorpha longicaudata. PLoS One. 2015;10:e0119619. Pedro SEM, Camargo JMF. Meliponini neotropicais: o gênero Partamona Schwarz, 1939 (Hymenoptera, Apidae). Rev Bras Entomol. 2003;47:1–117. Pinkel D, Straume T, Gray JW. Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci U S A. 1986;83:2934–8. Rambaut A: FigTree, ver. 1.3.1. (2009) [Online]. Available from: http://tree.bio.ed.ac.uk/software/figtree/. Last accessed 02/02/2017. Rambaut A, Drummond A: Tracer, Version 1.5. (2009). http://tree.bio.ed.ac.uk/software/tracer/. Last accessed 02/02/2017. Rasmussen C, Cameron SA. Global stingless bee phylogeny supports ancient divergence, vicariance, and long distance dispersal. Biol J Linn Soc. 2010;99:206–32. Ronquist F, Huelsenbeck JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003;19:1572–4. Ronquist F, Klopfstein S, Vilhelmsen L, Schulmeister S, Murray DL, Rasnitsyn AP. A total-evidence approach to dating with fossils, applied to the early radiation of the Hymenoptera. Syst Biol. 2012;61:973–99. Schweizer D. Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA-DAPI bands) in human chromosomes. Cytogenet Cell Genet. 1980;27:190–3. Sumner AT. A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res. 1972;75:304–6. Tamura Y, Harada Y, Nishikawa SI, Yamano K, Kamiya M, Shiota T, et al. Tam41 is a CDP-diacylglycerol synthase required for cardiolipin biosynthesis in mitochondria. Cell Metab. 2013;17:709–18. Tavares MG, Lopes DM, Campos LAO. An overview of cytogenetics of the tribe Meliponini (Hymenoptera: Apidae). Genetica. 2017;145:241–58. Tosta VC, Marthe JB, Tavares MG, Fernandes-Salomão TM, Pompolo SG, Recco-Pimentel SM, et al. Possible introgression of B chromosomes between bee species (genus Partamona). Cytogenet Genome Res. 2014;144:220–6. Travenzoli NM, Cardoso DC, Werneck HA, Fernandes-Salomão TM, Tavares MG, Lopes DM. The evolution of haploid chromosome numbers in Meliponini. PloS One. 2019;14:e0224463. Vaidya G, Lohman DJ, Meier R. SequenceMatrix: concatenation software for the fast assembly of multigene datasets with character set and codon information. Cladistics. 2011;27:171–80. Vicari MR, Noleto RB, Artoni RF, Moreira-Filho O, Bertollo LAC. Comparative cytogenetics among species of the Astyanax scabripinnis complex. Evolutionary and biogeographical inferences. Genet Mol Biol. 2008;31:173–9. Waldschmidt AM, Fernandes-Salomão TM, Barros EG, Campos LAO. Extraction of genomic DNA from Melipona quadrifasciata (Hymenoptera: Apidae, Meliponae). Braz J Genet. 1997;20:421–3. Article / Publication Details

First-Page Preview

Received: September 22, 2021
Accepted: October 28, 2021
Published online: December 17, 2021

Number of Print Pages: 9
Number of Figures: 3
Number of Tables: 2

ISSN: 1424-8581 (Print)
eISSN: 1424-859X (Online)

For additional information: https://www.karger.com/CGR

Copyright / Drug Dosage / Disclaimer Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher.
Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug.
Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.