J Appl Biomed 19:181-189, 2021 | DOI: 10.32725/jab.2021.019

Maryam Mansoori1, 2, Isa Abdi Rad3, Alireza Mirzaei4, Kevin J. Tam5, Seyed Mohsen Hosseini6, Rahim Mahmodlu7, Fatemeh Mansouri8, Leili Saeednejad Zanjani1, Zahra Madjd1, 2, * 1Iran University of Medical Sciences, Oncopathology Research Center, Tehran, Iran 2Iran University of Medical Sciences, Faculty of Advanced Technologies in Medicine, Department of Molecular Medicine, Tehran, Iran 3Urmia University of Medical Sciences, Cellular and Molecular Research Center, Urmia, Iran 4Iran University of Medical Sciences, Shafa Orthopedic Hospital, Bone and Joint Reconstruction Research Center, Tehran, Iran 5University of British Columbia, Vancouver Prostate Centre, Department of Urologic Sciences, Vancouver, Canada 6Omid specialty and subspecialty Hospital, Oncology and Radiotherapy Ward, Urmia, Iran 7Urmia University of Medical Sciences, Faculty of Medicine, Imam Khomeini Hospital, Department of Surgery, Urmia, Iran 8Urmia University of Medical Sciences, Faculty of Medicine, Department of Genetics and Immunology, Urmia, Iran

Introduction: Cancer stem cells (CSCs) are a theorized subset of cells within the tumor that is thought to drive disease recurrence and metastatic spread. The aim of this study is to investigate mRNA and protein levels of ganglioside GD2 synthase (GD2S), in breast cancer (BC) patients.

Methods: 65 PBMCs of preoperative BC patients without chemotherapy were compared to PBMCs after chemotherapy and controls.

Results: GD2S were significantly higher in BC patients after chemotherapy compared to pre-chemotherapy at both mRNA and protein. GD2S was higher in pre-chemotherapy blood samples compared to control samples.

Conclusions: Higher expression of GD2S in BC samples compared to healthy control indicates the potential utility of GD2S as a marker of malignancy.

Keywords: Breast cancer; Cancer stem cell; Circulating cancer stem cell; ELISA; qRT-PCR Grants and funding:

This study was supported by a grant from Iran University of Medical Sciences (Grant # 94-03-87-26452).

Conflicts of interest:

The authors report no conflict of interests.

Mansoori M, Abdi Rad I, Mirzaei A, Tam KJ, Mohsen Hosseini S, Mahmodlu R, et al.. Does GD2 synthase (GD2S) detect cancer stem cells in blood samples of breast carcinomas? J Appl Biomed. 2021;19(4):181-189. doi:10.32725/jab.2021.019.

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