The NF-κB pathway is critically implicated in the oncogenic phenotype of human osteosarcoma cells

J Appl Biomed 19:190-201, 2021 | DOI: 10.32725/jab.2021.021

Bingyi Tan, Zenong Yuan, Qingyu Zhang, Xu Xiqiang, Jun Dong * Shandong First Medical University, Shandong Provincial Hospital, Department of Orthopaedics, Jinan City, China

NF-κB is activated in a variety of human cancers. However, its role in osteosarcoma (OS) remains unknown. Here, we have elucidated the implication of NF-κB in the oncogenic phenotype of OS tumor cells. We reported that activation of NF-κB was a common event in the human OS. Inhibition of NF-κB using inhibitor Bay 11-7085 repressed proliferation, survival, migration, and invasion but increased apoptosis in 143B and MG63 OS cells, indicating that NF-κB is critically implicated in the oncogenesis of OS. Notably, Bay 11-7085 not only inactivated NF-κB but also reduced the phosphorylation of AKT via its induction of PTEN, suggesting the existence of a novel NF-κB/PTEN/PI3K/AKT axis. In vivo, Bay 11-7085 suppressed tumor growth in the bone by targeting NF-κB and AKT. Interestingly, combined treatment with Bay 11-7085 and the PI3K inhibitor, LY294002, triggered an augmented antitumor effect. Our results demonstrate that NF-κB potentiates the growth and aggressiveness of OS. Pharmacological inhibition of NF-κB represents a promising therapy for the treatment of OS.

Keywords: AKT; NF-κB; Osteosarcoma; Treatment Grants and funding:

This study was supported by the Shandong Provincial Medical Technology & Development Program (2019WS459) to J.D.

Conflicts of interest:

There is no competing interest related to the manuscript.

Tan B, Yuan Z, Zhang Q, Xiqiang X, Dong J. The NF-κB pathway is critically implicated in the oncogenic phenotype of human osteosarcoma cells. J Appl Biomed. 2021;19(4):190-201. doi:10.32725/jab.2021.021.

This is an open access article distributed under the terms of the Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.

留言 (0)

沒有登入
gif